Aberrant differential expression of EZH1 and EZH2 in Polycomb repressive complex 2 among B- and T/NK-cell neoplasms
Autor: | Hiaki Sato, Arie P. Otte, Katsuyoshi Takata, Tadashi Yoshino, Lamia Abdalkader, Ichiro Murakami, Takashi Oka |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Lymphoma B-Cell Cell Blotting Western macromolecular substances Biology Lymphoma T-Cell Pathology and Forensic Medicine 03 medical and health sciences medicine SUZ12 Biomarkers Tumor Humans Enhancer of Zeste Homolog 2 Protein EZH2 Polycomb Repressive Complex 2 Cell cycle medicine.disease Immunohistochemistry Haematopoiesis 030104 developmental biology medicine.anatomical_structure Cell culture Cancer research Mantle cell lymphoma |
Zdroj: | Pathology. 48(5) |
ISSN: | 1465-3931 |
Popis: | The Polycomb repressive complex-2 members (EZH2, EED, SUZ12 and EZH1) are important regulators of haematopoiesis, cell cycle and differentiation. Over-expression of EZH2 has been linked to cancer metastases and poor prognosis. Detailed information on the expression of other members in normal and neoplastic lymphoid tissue remains to be elucidated. Immunohistochemical and immunofluorescent analyses of 156 samples from haematopoietic neoplasms patients and 27 haematopoietic cell lines were used. B-cell neoplasms showed a significant over-expression of EZH2, EED and SUZ12 in the aggressive subtypes compared to the indolent subtypes and normal tissue (p = 0.000-0.046) while expression of EZH1 was decreased in mantle cell lymphoma compared to normal tissue (p = 0.011). T/NK-cell neoplasms also showed significant over-expression of EZH2, EED and SUZ12 (p = 0.000-0.002) and decreased expression of EZH1 (p = 0.001) compared to normal cells. EZH2 and EZH1 have opposite expression patterns both in normal and neoplastic lymphoid tissues as well as an opposite relation to Ki-67. These results were supported by western blotting analyses. Immunofluorescent staining revealed a difference in the intracellular localisation of EZH1 compared to other members. These evidences suggest that EZH2 and EZH1 are important in the counter-balancing mechanisms controlling proliferation/resting of lymphoid cells. The disruption of the balanced EZH2/EZH1 ratio may play important roles in the pathogenesis of lymphomas. |
Databáze: | OpenAIRE |
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