An arduous journey from human pluripotent stem cells to functional pancreatic β cells
| Autor: | Hwee Hui Lau, Chang Siang Lim, Joanita Binte Jasmen, Larry Sai Weng Loo, Adrian Kee Keong Teo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells Endocrinology Diabetes and Metabolism Cell Culture Techniques Biology Bioinformatics Models Biological 03 medical and health sciences Endocrinology Insulin-Secreting Cells Drug Discovery Insulin Secretion Internal Medicine medicine Animals Humans Hypoglycemic Agents Insulin Induced pluripotent stem cell Induced stem cells Cell Differentiation Embryonic stem cell In vitro Cell biology Endothelial stem cell Disease modelling 030104 developmental biology medicine.anatomical_structure Diabetes Mellitus Type 1 Diabetes Mellitus Type 2 Beta cell Pancreas |
| Zdroj: | Diabetes, obesitymetabolism. 20(1) |
| ISSN: | 1463-1326 |
| Popis: | Type 1 and type 2 diabetes are caused by a destruction and decrease in the number of functional insulin-producing β cells, respectively; therefore, the generation of functional β cells from human embryonic stem cells and human induced pluripotent stem cells, collectively known as human pluripotent stem cells (hPSCs), for potential cell replacement therapy and disease modelling is an intensely investigated area. Recent scientific breakthroughs enabled derivation of large quantities of human pancreatic β-like cells in vitro, although with varied glucose-stimulated insulin secretion kinetics. In the present review, we comprehensively summarize, compare and critically analyze the intricacies of these developing technologies, including differentiation platforms, robustness of protocols, and methodologies used to characterize hPSC-derived β-like cells. We also discuss experimental issues that need to be resolved before these β-like cells can be used clinically. |
| Databáze: | OpenAIRE |
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