Transforming growth factor-β inhibits nephronectin-induced osteoblast differentiation
| Autor: | Chengyan He, Weining Yang, Arun Seth, Chun Peng, Shireen Kahai, Burton B. Yang, Ling Fang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
TGF-β
Blotting Western Biophysics Nephronectin Matrix (biology) Cell morphology Biochemistry Cell Line Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Structural Biology Transforming Growth Factor beta Gene expression Genetics medicine Animals Molecular Biology 030304 developmental biology 0303 health sciences Extracellular Matrix Proteins Osteoblasts biology Matrix Reverse Transcriptase Polymerase Chain Reaction Osteoblast Cell Differentiation Cell Biology Transfection Transforming growth factor beta Molecular biology medicine.anatomical_structure Phosphodiesterase I 030220 oncology & carcinogenesis biology.protein Transforming growth factor |
| Zdroj: | FEBS Letters. (13):2877-2882 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2010.04.074 |
| Popis: | We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We found that the effect of TGF-beta on Npnt expression is associated with a change in cell morphology in a dose-dependent manner. Npnt-induced osteoblast differentiation was also inhibited by TGF-beta, which changed cell morphology from cuboidal to fibroblastic, an indication that osteoblast differentiation was disrupted. Furthermore, TGF-beta inhibited differentiation of osteoblasts transfected with various truncated Npnt constructs, suggesting that TGF-beta can exert a down-stream effect on Npnt function. Our results suggest that TGF-beta can inhibit osteoblast differentiation through various mechanisms. |
| Databáze: | OpenAIRE |
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