First Case of NDM-1-Producing Klebsiella pneumoniae in Annaba University Hospital, Algeria
| Autor: | Sabina Nedjai, Nassima Djahmi, Mazouz Dekhil, Jean-Philippe Lavigne, Alix Pantel, Mohamed Cherif Bentakouk, Albert Sotto, Charlotte Pujol, Djamila Kirane-Gacemi, Amel Abderrahim |
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| Přispěvatelé: | Virulence bactérienne et maladies infectieuses (VBMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Badji Mokhtar - Annaba [Annaba] (UBMA), CHU Ibn Rochd [Casablanca], Centre Hospitalier Universitaire de Nîmes (CHU Nîmes) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Ertapenem Male Klebsiella pneumoniae medicine.drug_class 030106 microbiology Immunology Antibiotics Repetitive Sequences Gene Expression Microbial Sensitivity Tests NDM-1 beta-Lactams Microbiology beta-Lactamases Hospitals University 03 medical and health sciences Quinolone resistance chemistry.chemical_compound carbapenemase [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Drug Resistance Multiple Bacterial medicine Humans Phylogeny Pharmacology biology IncR biology.organism_classification University hospital ST147 3. Good health Anti-Bacterial Agents Clone Cells Klebsiella Infections [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Algeria [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology Multilocus sequence typing Multilocus Sequence Typing Plasmids MLST |
| Zdroj: | Microbial Drug Resistance Microbial Drug Resistance, Mary Ann Liebert, 2017, 23 (7), pp.895-900. ⟨10.1089/mdr.2016.0213⟩ |
| ISSN: | 1076-6294 |
| DOI: | 10.1089/mdr.2016.0213⟩ |
| Popis: | International audience; AIM:The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014.RESULTS:Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive. The two isolates harbored the blaNDM-1 gene and one was also positive for blaCTX-M-15. Screening of aminoglycoside-modifying enzymes and plasmid-mediated quinolone resistance contents detected aac(6')-Ib-cr, aac(3')-II, qnrB2, and oqxAB in both isolates. Multilocus sequence typing demonstrated that the two isolates belonged to sequence type 147. However, repetitive sequence-based PCR and pulsed-field gel electrophoresis showed that they were not clonally related. The blaNDM-1 gene and all other resistant genes were contained on an IncR plasmid of c.a. 85 kb.CONCLUSIONS:This study comprises the first identification of NDM-1-producing K. pneumoniae in Algeria. We thus confirm the concerning worldwide dissemination of this carbapenemase that involves the emergence of the IncR plasmid and the success of the ST147 clonal complex harboring it. |
| Databáze: | OpenAIRE |
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