Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts
| Autor: | R.W. Goldbach, A. C. de Ávila, E. W. Kitajima, R. de O. Resende, Dirk Peters |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Antiserum
biology Inoculation Immunoelectron microscopy Laboratory of Virology Immunogold labelling Plant cell Immunohistochemistry Virology Virus Plant Viruses Microbiology Tomato spotted wilt virus Laboratorium voor Virologie Capsid Fruit Defective particle biology.protein Antibody Microscopy Immunoelectron Protein A Plant Diseases |
| Zdroj: | Journal of Virological Methods 38 (1992) Journal of Virological Methods, 38, 313-322 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(92)90076-p |
| Popis: | Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. |
| Databáze: | OpenAIRE |
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