Intestinal flora imbalance affects bile acid metabolism and is associated with gallstone formation
| Autor: | Haifeng Lu, Bei Sun, Weihui Zhang, Chenjun Hao, Qiang Wang, Dongbo Xue, Biao Ma, Long Li, Defu Zhu, Wenchao Yao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult DNA Bacterial Male Firmicutes medicine.drug_class Gene Expression Gut microbiota Bile acid BSH Gallstones Gut flora digestive system Microbiology Bile Acids and Salts 03 medical and health sciences Feces 0302 clinical medicine Ruminococcus gnavus RNA Ribosomal 16S Enterohepatic Circulation Medicine Humans lcsh:RC799-869 Enterohepatic circulation 16S rRNA gene sequencing biology Bacteria business.industry Sequence Analysis RNA Gastroenterology General Medicine Middle Aged biology.organism_classification medicine.disease Gastrointestinal Microbiome 030104 developmental biology Cholesterol Gallstone Dysbiosis 030211 gastroenterology & hepatology lcsh:Diseases of the digestive system. Gastroenterology Female business Research Article |
| Zdroj: | BMC Gastroenterology BMC Gastroenterology, Vol 20, Iss 1, Pp 1-13 (2020) |
| Popis: | BackgroundThe gut microbiota participates in the metabolism of substances and energy, promotes the development and maturation of the immune system, forms the mucosal barrier, and protects the host from pathogen attacks. Although the pathogenesis of cholesterol gallstones is still not clear, studies have suggested that gut microbiota dysbiosis plays an important role in their formation.MethodsMicrobial DNA from faeces of normal control patients and those of patients with calculi was subjected to 16S rRNA gene sequencing to detect gene expression changes in intestinal microbes. ELISA kits were used to measure free bile acids, secondary bile acids and coprostanol according to the manufacturer’s instructions. The relationship between flora and their metabolites was then analysed.ResultsIn the gallstone group, the diversity of intestinal bacteria and the abundances of certain phylogroups were significantly decreased (p Firmicutes(p p p p p Ruminococcus gnavusgroup could be used as a biomarker to distinguish the gallstone group from the control group.ConclusionWe conclude that intestinal flora imbalance affects bile acid and cholesterol metabolism and is associated with gallstone formation. |
| Databáze: | OpenAIRE |
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