Determination of the illicit drug gamma-hydroxybutyrate (GHB) in human saliva and beverages by1H NMR analysis
| Autor: | Christopher J.L. Silwood, Martin Grootveld, A.D. Clark, Deborah Algeo, John C. Blackburn |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
chemistry.chemical_classification Saliva Magnetic Resonance Spectroscopy Chromatography Molecular Structure Clinical Biochemistry Analytical chemistry Reproducibility of Results NMR tube High resolution Gamma hydroxybutyrate General Medicine Nuclear magnetic resonance spectroscopy Biochemistry Beverages Substance Abuse Detection chemistry Propionate Proton NMR Humans Molecular Medicine Illicit drug Sodium Oxybate |
| Zdroj: | BioFactors. 27:121-136 |
| ISSN: | 1872-8081 0951-6433 |
| Popis: | High resolution 1H NMR spectroscopy has been employed to investigate the detection and quantification of the illicit "date-rape" drug gamma-hydroxybutyrate (GHB) in both human saliva and a commonly-consumed low-alcohol beer product. Data acquired revealed that this multicomponent analytical technique provided unequivocal evidence for the detection of this agent by this technique in both of these matrices, i.e., all three of its resonances [those ascribable to the alpha-CH2 (t, delta=2.25 ppm), beta-CH2 (tt, delta=1.81 ppm) and gamma-CH2 (t, delta=3.61 ppm) group protons] were present in spectra acquired on human saliva, and two of these (the alpha- and beta-CH2 group signals) in the beverage product examined, the latter observation attributable to overlap of the gamma-CH2 1H resonance with those of carbohydrates. Since good linear calibration relationships between the intensities of each of the NMR-visible signals and added GHB concentration (the former normalised to that of an external 3-trimethylsilyl [2,2,3,3-2H4]- propionate standard present in a coaxial NMR tube insert) were observed, this illicit drug is also readily quantifiable in such multicomponent samples. Our data demonstrate the advantages offered by this technique when applied to the analysis of illicit drugs in multicomponent sample matrices such as human biofluids and beverage products. |
| Databáze: | OpenAIRE |
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