Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques
| Autor: | Hana Nevídalová, Lenka Michalcová, Zdeněk Glatz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Systems biology
Clinical Biochemistry 02 engineering and technology Computational biology Mass spectrometry 01 natural sciences Biochemistry Chromatography Affinity Mass Spectrometry Analytical Chemistry Poor concentration Electrophoresis Microchip Capillary electrophoresis Humans chemistry.chemical_classification Laboratory methods Biomolecule 010401 analytical chemistry Electrophoresis Capillary Blood Proteins Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Interaction studies Spectrometry Fluorescence chemistry Nucleic acid Peptides 0210 nano-technology |
| Zdroj: | ELECTROPHORESIS. 40:625-642 |
| ISSN: | 0173-0835 |
| Popis: | Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides, and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics, and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterized by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method-ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry, and several nontraditional detection techniques in affinity interaction studies are summarized and discussed, together with the possibility of conducting these measurements in microchip format. |
| Databáze: | OpenAIRE |
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