Development and application of criteria for classification of hydrolysed proteins in the framework of feed safety
| Autor: | Leo W. D. van Raamsdonk, Christophe Genouel, Anna Weiner, Theo W. Prins, Nelly Jardy, Stanislava Vonsovic, Ioana M. Barbu, Marilyne Bescond, Ilona Paprocka, Krzysztof Kwiatek |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Minerals
animal feed Team Animal Drugs 1 Health Toxicology and Mutagenesis Business Unit Microbiology & Agrochains Public Health Environmental and Occupational Health Proteins Water processed animal proteins General Chemistry General Medicine Business Unit Microbiologie & Agroketens Toxicology Animal Feed Team Growth Promotors Solvents Animals hydrolysation index Team Virology & GMO Peptides Animal protein hydrolysates Food Science |
| Zdroj: | Food Additives and Contaminants-Part A 39 (2022) 10 Food Additives and Contaminants-Part A, 39(10), 1674-1690 |
| ISSN: | 1944-0049 |
| Popis: | In the view of a circular economy, there is an increasing need for (re-)using animal by-products that have a wide range of applications and sufficient safety. Hydrolysates of animal proteins (HPs) are frequently used as feed ingredients. Nevertheless, clear criteria for legal use and methods for monitoring feed applications are not available. Here, a range of methods have been used and evaluated for characterizing a set of 26 samples of hydrolysed proteins, ‘hydrolysed’ feather meals and processed animal proteins (PAPs), with verification based on an additional set of eight samples. Methods included determination of ash content, sediment (mineral fraction) content, protein content, species identity, solubility, protein solubility, size exclusion chromatography and polyacrylamide gel electrophoresis (SDS-PAGE). After a comparison of results obtained with water and SDS, water was chosen as the solvent for environmental and occupational reasons. Typical HP samples have a protein content higher than 60%, a solubility exceeding 50% and a virtual absence of a mineral fraction. The first discrimination between HPs and PAPs could be based on the absence or presence, respectively, of a mineral fraction. An approach for HP characterization is designed using a Hydrolysation Index (HI) based on the fraction of peptides smaller than 10 kDa, the solubility of the sample and the fraction of soluble proteins. A simplified version (HIs), exclusively based on the fraction of peptides smaller than 10 kDa and the solubility of the sample, shows a trend among the samples highly comparable to HI. Values for HI and HIs exceeding 60% would characterise HPs. Feather meals, which are heat treated instead of treatment by a chemical process of hydrolysation, range among the PAPs and should not be indicated as “hydrolysed.” The HIs can be used as an easy parameter for classifying HPs and for legal enforcement. |
| Databáze: | OpenAIRE |
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