Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces
| Autor: | David L. Cochran, Joseph K. Williams, Barbara D. Boyan, Michael B. Berger, David J. Cohen, Rene Olivares-Navarrete, Zvi Schwartz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.drug_class Surface Properties 1 25-Dihydroxy vitamin D3 Osteocalcin lcsh:Medicine Biocompatible Materials 02 engineering and technology Osseointegration lcsh:Physiology Gender Studies 03 medical and health sciences Endocrinology Osteoprotegerin Internal medicine medicine Humans Vitamin D Cells Cultured Titanium Sex Characteristics Osteoblasts biology lcsh:QP1-981 Estradiol Chemistry Research lcsh:R Osteoblast Estrogens 021001 nanoscience & nanotechnology Hormones Sexual dimorphism 030104 developmental biology medicine.anatomical_structure Estrogen biology.protein Alkaline phosphatase Sex-specific responses 0210 nano-technology Human osteoblasts Titanium surface roughness Transforming growth factor |
| Zdroj: | Biology of Sex Differences Biology of Sex Differences, Vol 9, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2042-6410 |
| Popis: | Background Osseointegration is dependent on the implant surface, surrounding bone quality, and the systemic host environment, which can differ in male and female patients. Titanium (Ti) implants with microstructured surfaces exhibit greater pullout strength when compared to smooth-surfaced implants and exhibit enhanced osteogenic cellular responses in vitro. Previous studies showed that 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] has a greater effect on rat osteoblast differentiation on microstructured Ti compared to smooth Ti surfaces and tissue culture polystyrene (TCPS). The stimulatory effect of 17β-estradiol (E2) on differentiation is observed in female osteoblasts on micro-rough Ti, but it is not known if male osteoblasts behave similarly in response to E2 and microtopography. This study assessed whether human male and female osteoblasts exhibit sex-specific differences in response to E2 and 1α,25(OH)2D3 when cultured on microstructured Ti surfaces. Methods Osteoblasts from three male and three female human donors were cultured on Ti discs with varying surface profiles: a smooth pretreatment (PT), a coarse grit-blasted/acid-etched (SLA), and an SLA surface having undergone modification in a nitrogen environment and stored in saline to maintain hydrophilicity (modSLA). Cells cultured on these surfaces were treated with E2 or 1α,25(OH)2D3. Results Male and female human osteoblasts responded similarly to microstructure although there were donor-specific differences; cell number decreased, and osteocalcin (OCN), osteoprotegerin (OPG), and latent and active transforming growth factor 1 increased on SLA and modSLA compared to TCPS. Female osteoblasts had higher alkaline phosphatase activity and OCN production than male counterparts but produced less OPG. Both sexes responded similarly to 1α,25(OH)2D3. E2 treatment reduced cell number and increased osteoblast differentiation and factor production only in female cells. Conclusions Male and female human osteoblasts respond similarly to microstructure and 1α,25(OH)2D3 but exhibit sexual dimorphism in substrate-dependent responses to E2. E2 affected female osteoblasts, suggesting that signaling is sex-specific and surface-dependent. Donor osteoblasts varied in response, demonstrating the need to test multiple donors when examining human samples. Understanding how male and female cells respond to orthopedic biomaterials will enable greater predictability post-implantation as well as therapies that are more patient-specific. Electronic supplementary material The online version of this article (10.1186/s13293-018-0190-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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