The differentiation of oral soft- and hard tissues using laser induced breakdown spectroscopy - a prospect for tissue specific laser surgery
| Autor: | Fanuel Mehari, Florian Stelzle, Michael Schmidt, Florian Klämpfl, Maximilian Rohde, Werner Adler, Friedrich-Wilhelm Neukam |
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| Rok vydání: | 2016 |
| Předmět: |
Laser surgery
Pathology medicine.medical_specialty Materials science Swine medicine.medical_treatment General Physics and Astronomy 01 natural sciences General Biochemistry Genetics and Molecular Biology 010309 optics stomatognathic system 0103 physical sciences medicine Animals Tissue specific General Materials Science Laser-induced breakdown spectroscopy Laser beams Enamel paint 010401 analytical chemistry General Engineering General Chemistry 0104 chemical sciences stomatognathic diseases medicine.anatomical_structure Organ Specificity visual_art Principal component analysis visual_art.visual_art_medium Pulp (tooth) Laser Therapy Cancellous bone Biomedical engineering |
| Zdroj: | Journal of Biophotonics. 10:1250-1261 |
| ISSN: | 1864-063X |
| Popis: | Compared to conventional techniques, Laser surgery procedures provide a number of advantages, but may be associated with an increased risk of iatrogenic damage to important anatomical structures. The type of tissue ablated in the focus spot is unknown. Laser-Induced Breakdown-Spectroscopy (LIBS) has the potential to gain information about the type of material that is being ablated by the laser beam. This may form the basis for tissue selective laser surgery. In the present study, 7 different porcine tissues (cortical and cancellous bone, nerve, mucosa, enamel, dentine and pulp) from 6 animals were analyzed for their qualitative and semiquantitative molecular composition using LIBS. The so gathered data was used to first differentiate between the soft- and hard-tissues using a Calcium-Carbon emission based classifier. The tissues were then further classified using emission-ratio based analysis, principal component analysis (PCA) and linear discriminant analysis (LDA). The relatively higher concentration of Calcium in the hard tissues allows for an accurate first differentiation of soft- and hard tissues (100% sensitivity and specificity). The ratio based statistical differentiation approach yields results in the range from 65% (enamel-dentine pair) to 100% (nerve-pulp, cancellous bone-dentine, cancellous bone-enamel pairs) sensitivity and specificity. Experimental LIBS measuring setup. |
| Databáze: | OpenAIRE |
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