Evaluation of the diagnostic accuracy of the modified agglutination test (MAT) and an indirect ELISA for the detection of serum antibodies against Toxoplasma gondii in sheep through Bayesian approaches
| Autor: | M. Barberán, Raúl C. Mainar-Jaime |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Veterinary medicine
Veterinary parasitology Antibodies Protozoan Sheep Diseases Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Serology Species Specificity Pregnancy Agglutination Tests Direct agglutination test parasitic diseases Credible interval medicine Animals Sheep General Veterinary biology Reproducibility of Results Toxoplasma gondii Bayes Theorem General Medicine Abortion Veterinary medicine.disease biology.organism_classification Toxoplasmosis Confidence interval Toxoplasmosis Animal Animals Newborn Immunology biology.protein Female Parasitology Antibody Toxoplasma |
| Zdroj: | Veterinary Parasitology. 148:122-129 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2007.05.018 |
| Popis: | The diagnostic accuracies of the modified agglutination test (MAT) and indirect ELISA test for the detection of serum antibodies against Toxoplasma gondii in sheep were evaluated through Bayesian approaches on two populations of sheep created from three different groups of animals (T. gondii-aborted ewes, colostrums-deprived newborn lambs, and ewe-lambs and adult ewes with unknown T. gondii infection status). Tests showed a high degree of agreement (kappa statistic = 0.93; 95% confidence interval = 0.87, 0.98) and a significant specificity (Sp) correlation (gamma(Sp) = 0.26; 95% credibility interval = 0.017, 0.61). When prior information was used for all unknown parameters the posterior medians for the sensitivity (Se) and Sp of the MAT and ELISA were, respectively, 92.6% (95% credibility interval = 85.2, 96.9), 95.5% (89.9, 98.7), 90.5% (83.4, 95.6), and 97.8% (94.2, 99.5). These estimates remained similar when uninformative priors were included. The Se estimates of the MAT and ELISA were higher than those obtained on pigs in other study using the same approach (Se = 80.6% and Sp = 89.5% for the MAT, and Se = 71.5% and Sp = 85.5% for the ELISA [Georgiadis, M.P., Wesley, O.J., Gardner, I.A., Singh, R., 2003. Correlation-adjusted estimation of sensitivity and specificity of two diagnostic tests. Appl. Stat. 52, 63-78]. This finding supported the believe that test performances may vary when applied on different animal species. Thus, if these tests are planned to be used on animal species other than sheep or pigs, their diagnostic accuracy should be re-assessed to prevent biased inferences from their results. |
| Databáze: | OpenAIRE |
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