Bioanalytical applications of capillary electrophoresis with laser-induced native fluorescence detection
| Autor: | Hua-Ming Tseng, Yin Li, David A. Barrett |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Analyte
Bioanalysis Chromatography Chemistry Lasers Clinical Biochemistry Analytic Sample Preparation Methods Electrophoresis Capillary Clinical Chemistry Tests General Medicine Mass spectrometry Fluorescence Analytical Chemistry Medical Laboratory Technology Electrophoresis chemistry.chemical_compound Spectrometry Fluorescence Capillary electrophoresis Humans General Pharmacology Toxicology and Pharmaceutics Derivatization |
| Zdroj: | Bioanalysis. 2:1641-1653 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.10.72 |
| Popis: | In this article we describe recent developments and applications of capillary electrophoresis (CE) coupled with laser-induced native fluorescence (LINF) detection in the analysis of biological, pharmaceutical and environmental samples. Compared with traditional UV absorbance detection used in CE, the LINF technique can greatly improve the concentration sensitivity of CE without the need for derivatization; the only requirement being that the analyte must have native fluorescence. Instrumentation and laser sources used in CE–LINF are summarized and specific applications of CE–LINF to small-biomolecule analysis, profiling of human biofluids, detection of native fluorescent peptides and proteins, single-cell analysis and the use of online sample preconcentration methods are also reviewed in detail. |
| Databáze: | OpenAIRE |
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