The design of antimicrobial LL37-modified collagen-hyaluronic acid detachable multilayers
| Autor: | Padmavathy Rajagopalan, Sophia M. Orbach, Margaret E. Cassin, Andrew J. Ford, Scott E. Saverot |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Materials science Antimicrobial peptides Biomedical Engineering Peptide 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Bacterial Adhesion Microbiology Biomaterials chemistry.chemical_compound Coated Materials Biocompatible Cathelicidins Hyaluronic acid Escherichia coli medicine Animals Humans Hyaluronic Acid Cytotoxicity Molecular Biology Cells Cultured chemistry.chemical_classification Membranes Artificial General Medicine Adhesion 021001 nanoscience & nanotechnology Antimicrobial Rats 0104 chemical sciences Membrane chemistry Rats Inbred Lew Hepatocytes Collagen 0210 nano-technology Antimicrobial Cationic Peptides Biotechnology |
| Zdroj: | Acta Biomaterialia. 40:119-129 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2016.04.027 |
| Popis: | The design of antimicrobial membranes and thin films are critical for the design of biomaterials that can combat bacterial contamination. Since the long-term use of conventional antibiotics can result in bacterial resistance, there is a critical need to incorporate natural antimicrobial peptides (AMPs) that not only prevent a wide range of pathogens from causing infections but can also promote many beneficial outcomes in wounded tissues. We report the design and antimicrobial properties of detachable collagen (COL)/hyaluronic acid (HA) polyelectrolyte multilayers (PEMs) modified with LL-37, a naturally occurring human AMP. LL-37 was physically adsorbed and chemically immobilized on the surface of PEMs. The antimicrobial and cytotoxic properties of PEMs were tested with Gram-negative Escherichia coli (E. coli, strain DH10B) and primary rat hepatocytes, respectively. The ability to prevent bacterial adhesion and to neutralize an E. coli layer was investigated as a function of LL-37 concentration. An interesting trend was that even unmodified PEMs exhibited a 40% reduction in bacterial adhesion. When LL-37 was physically adsorbed on PEMs, bacterial adhesion was significantly lower on the surface of the films as well as in the surrounding broth. Immobilizing LL-37 resulted in less than 3% bacterial adhesion on the surface due to the presence of the peptide. LL-37 modified PEMs did not result in any cytotoxicity up to input concentrations of 16 μM. More importantly, urea and albumin secretion by hepatocytes were unaffected even at high LL-37 concentrations. The COL/HA PEMs can serve as antimicrobial coatings, biological membranes and as in vitro platforms to investigate pathogen-tissue interactions. Statement of Significance Antimicrobial peptides (AMPs) are emerging as an alternative to conventional antibiotics. We report the antimicrobial properties of detachable collagen (COL)/hyaluronic acid (HA) polyelectrolyte multilayers (PEMs) modified with LL-37, a human AMP. The antimicrobial and cytotoxic properties were tested with gram-negative Escherichia coli (E. coli, strain DH10B) and primary rat hepatocytes, respectively. Unmodified PEMs exhibited a 40% reduction in bacterial adhesion. When LL-37 was physically adsorbed on PEMs, the sustained release of the active peptide killed planktonic bacteria. Immobilizing LL-37 resulted in less than 3% bacterial adhesion. LL-37 modified PEMs did not result in cytotoxicity up to input concentrations of 16 μM. The COL/HA PEMs can serve as antimicrobial coatings and to investigate pathogen-cell interactions. |
| Databáze: | OpenAIRE |
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