Efficacy of an Escherichia coli O157:H7 SRP Vaccine in Orally Challenged Goats and Strain Persistence Over Time
| Autor: | Hannah J. Sylvester, Jacob M Swift, A. T. Rogers, Derek M. Foster, Emily H. Griffith, Megan E. Jacob |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine 030106 microbiology 030231 tropical medicine Administration Oral Porins Food Contamination Receptors Cell Surface Biology Escherichia coli O157 medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Persistence (computer science) Feces 03 medical and health sciences 0302 clinical medicine Pulsed-field gel electrophoresis medicine Animals Escherichia coli Escherichia coli Infections Goat Diseases Strain (chemistry) Escherichia coli Vaccines business.industry Escherichia coli Proteins Goats Vaccination Outbreak Electrophoresis Gel Pulsed-Field Red Meat Milk Food Microbiology Animal Science and Zoology Livestock business Bacterial Outer Membrane Proteins Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 14:160-166 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | Small ruminants have been implicated in outbreaks of Escherichia coli O157:H7 at livestock exhibitions throughout the United States. Additionally, goat meat or milk may serve as a reservoir for foodborne transmission of the organism. These associations highlight the public health importance of an effective strategy to reduce E. coli O157:H7 shedding in goats. We examined the efficacy of the SRP® vaccine in goats orally challenged with E. coli O157:H7. Mixed-breed goats (n = 14) were randomly allocated into vaccinated and unvaccinated treatments (n = 7 per treatment). Goats were housed with a vaccinated and unvaccinated animal in each pen. Feces were collected for 3 weeks, then at necropsy, gastrointestinal contents were collected to determine the concentration of E. coli O157:H7. Three isolates per positive sample were saved and evaluated by pulsed-field gel electrophoresis (PFGE) to assess strain persistence over time. The mean concentration of E. coli O157:H7 in the feces of goats was numerical... |
| Databáze: | OpenAIRE |
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