Stearoyl-CoA desaturase 1 coding sequences abd antisense RNA affect lipid secretion in transfected chicken LMH hepatoma cells
| Autor: | Pascal Lefevre, Jean-Pierre Poisson, Michel Narce, C. Diot, Bénédicte Belloir, Christophe Hervé, M. Douaire, Jacques Mallard, Marie Damon |
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| Přispěvatelé: | Génétique Animale (GARen), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, Station de recherches porcines, Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
animal structures
[SDV]Life Sciences [q-bio] Biophysics Gene Expression Adipose tissue Biology Transfection Biochemistry 03 medical and health sciences Liver Neoplasms Experimental Gene expression Tumor Cells Cultured Animals RNA Antisense RNA Messenger RNA Neoplasm Molecular Biology ComputingMilieux_MISCELLANEOUS DNA Primers 030304 developmental biology 0303 health sciences Expression vector Base Sequence 030302 biochemistry & molecular biology nutritional and metabolic diseases RNA Lipid Metabolism Molecular biology Recombinant Proteins Antisense RNA Isoenzymes Stearoyl-CoA Desaturase Liver embryonic structures lipids (amino acids peptides and proteins) Stearoyl-CoA desaturase-1 Chickens |
| Zdroj: | Archives of Biochemistry and Biophysics Archives of Biochemistry and Biophysics, Elsevier, 2000, 380 (2), pp.243-250 |
| ISSN: | 0003-9861 1096-0384 |
| Popis: | Hepatic stearoyl CoA desaturase (SCD) activity in chickens from a fat line is higher than that of chickens from a lean line and correlates with plasma triacylglycerol concentrations. Furthermore, in these lines, the hepatic SCD1 mRNA level is positively correlated with the adipose tissue weight. To analyze the contribution of the SCD1 gene in the regulation of adiposity in the early stages of triacylglycerol secretion, SCD1 coding sequence and antisense RNA expression vectors were transfected in LMH cells. After selection, these cells were analyzed with regard to SCD1 expression and lipid secretion. The amounts of secreted triacylglycerols and phospholipids were shown to be higher in LMH cells transfected with the SCD1 gene, but reduced in those transfected with the SCD1 antisense sequences when compared to cells transfected with the vector alone (without SCD1 sequences). These results provide direct evidence that the expression of the SCD1 gene plays a major role in the triacylglycerol and phospholipid secretion process. |
| Databáze: | OpenAIRE |
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