Intranasal Immunization with Mumps Virus DNA Vaccine Delivered by Influenza Virosomes Elicits Mucosal and Systemic Immunity
| Autor: | Rinaldo Zurbriggen, Reinhard Glück, Marco Donati, Peter Durrer, Marcello Valassina, Silvia Bianchi, Maria Grazia Cusi, Pier Egisto Valensin |
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| Rok vydání: | 2000 |
| Předmět: |
influenza virosome
DNA vaccine Orthomyxoviridae Mumps Vaccine Hemagglutinin (influenza) Mumps virus Antibodies Viral medicine.disease_cause Virus DNA vaccination Mice Th2 Cells Immune system Virology Chlorocebus aethiops Vaccines DNA medicine Animals Immunity Mucosal Mumps Vero Cells Administration Intranasal Mice Inbred BALB C biology Gene Transfer Techniques Th1 Cells biology.organism_classification Interleukin-10 Immunization Antibody Formation Immunology biology.protein Female mumps virus Nasal administration |
| Zdroj: | Università degli Studi di Siena-IRIS |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0605 |
| Popis: | To improve the efficiency of liposome-mediated DNA transfer as a tool for gene therapy or vaccinology, we have further developed a new delivery system based on the modified immunopotentiating reconstituted influenza virus (IRIV). In this study, we engineered a plasmid DNA vector expressing the mumps virus hemagglutinin or the fusion protein. The administration of this DNA vaccine delivered by influenza virosomes, in combination with the mucosal adjuvant Escheriagen via the intranasal route, was efficient for inducing an immune response, both mucosally and systemically, in mice. The production of IgG2a mumps virus-specific antibodies and the secretion of interleukin 10 (IL-10) by antigen-specific T cells indicated that not only Th1 but also Th2 responses were induced by this DNA vaccine formulation. These results suggest that cationic virosomes in combination with Escheriagen may have great potential as an efficient delivery system for intranasal DNA immunization and provide an immune barrier at the mucosal sites. |
| Databáze: | OpenAIRE |
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