Electro-responsive graphene oxide hydrogels for skin bandages: The outcome of gelatin and trypsin immobilization

Autor: Mariagrazia Di Luca, Florida Voli, Giuseppe Cirillo, Silke Hampel, Manuela Curcio, Orazio Vittorio, Magdalena Czuban, Fiore Pasquale Nicoletta, Francesca Iemma, Annafranca Farfalla
Rok vydání: 2018
Předmět:
Pharmaceutical Science
02 engineering and technology
Spectrum Analysis
Raman
01 natural sciences
Gelatin
Polyethylene Glycols
Polymerization
chemistry.chemical_compound
Trypsin
Raman
Graphene oxide
chemistry.chemical_classification
Acrylamide
Hybrid hydrogels
Hydrogels
Oxides
Polymer
021001 nanoscience & nanotechnology
Thermogravimetry
Self-healing hydrogels
Methacrylates
Graphite
0210 nano-technology
Methicillin-Resistant Staphylococcus aureus
Curcumin
food.ingredient
Biocompatibility
Immobilized enzyme
Cell Survival
Radical polymerization
Polyethylene glycol
010402 general chemistry
Electro-responsive release
Cell Line
food
Tensile Strength
Enzyme immobilization
Humans
Antimicrobial
Drug Liberation
Fibroblasts
Bandages
3003
Spectrum Analysis
technology
industry
and agriculture
0104 chemical sciences
chemistry
Nuclear chemistry
Zdroj: International Journal of Pharmaceutics. 546:50-60
ISSN: 0378-5173
Popis: A free radical polymerization method was adopted for the fabrication of hybrid hydrogel films based on acrylamide and polyethylene glycol dimethacrylate as plasticizing and crosslinking agents, respectively, to be employed as smart skin bandages. Electro-sensitivity, biocompatibility and proteolytic properties were conferred to the final polymer networks by introducing graphene oxide (0.5% w/w), gelatin or trypsin (10% w/w) in the polymerization feed. The physical chemical and mechanical characterization of hybrid materials was performed by means of determination of protein content, Raman spectroscopy, thermogravimetric analysis and measurement of tensile strength. The evaluation of both water affinity and curcumin release profiles (analyzed by suitable mathematical modelling) upon application of an external electric stimulation in the 0-48 voltage range, confirmed the possibility to modulate the release kinetics. Proper proteolytic tests showed that the trypsin enzymatic activity was retained by 80% upon immobilization. Moreover, for all samples, we observed a viability higher than 94% in normal human fibroblast cells (MRC-5), while a reduction of methicillin-resistant Staphylococcus aureus CFU mL-1 (90%) was obtained with curcumin loaded samples.
Databáze: OpenAIRE
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