Impaired immunoregulatory network of the CD4 T lymphocytes in refractory asthma
| Autor: | Lorena S. Andrade, Tarcísio S. Almeida, Maria Ilma Araujo, Luciana Santos Cardoso, Alvaro A. Cruz, Edgar M. Carvalho, Diego Mota Lopes, Luane Marques de Mello, Jamille Souza Fernandes |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult CD4-Positive T-Lymphocytes Hypersensitivity Immediate Male Refractory period Immunology Population Peripheral blood mononuclear cell Immunophenotyping Atopy Immunomodulation 03 medical and health sciences 0302 clinical medicine T-Lymphocyte Subsets Immunology and Allergy Medicine Humans Clinical significance CTLA-4 Antigen Age of Onset Vitamin D education Asthma education.field_of_study business.industry FOXP3 T lymphocyte Immunoglobulin E Middle Aged medicine.disease Flow Cytometry respiratory tract diseases Respiratory Function Tests body regions 030104 developmental biology 030228 respiratory system Leukocytes Mononuclear Cytokines Female business Biomarkers |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 49(5) |
| ISSN: | 1365-2222 |
| Popis: | Background The immunopathogenesis of severe asthma has been associated with an inefficient regulatory response. There are a few studies about the CD4 T cells profile among individuals with severe asthma refractory to treatment. Objective To evaluate the CD4 T lymphocyte profile from individuals with severe asthma according to their response to treatment, relating to their atopy status and age of asthma onset. Methods We evaluated nineteen individuals with severe asthma refractory to treatment (SAR), 21 with well-controlled or partly controlled severe asthma (CSA) and 23 with mild-to-moderate asthma (MMA). Lymphocytes were obtained from PBMC, and the frequency of expression of different molecules in this population was assessed using the flow cytometry. Results We observed the frequency of CD4+ IFN-γ+ T cells was higher in atopic individuals with SAR than with CSA. In addition, among the atopic and early-onset asthma (EOA), the frequency of CD4+ CTLA-4+ T cells was lower in the SAR group than the CSA group. In relation to non-atopic and late-onset asthma (LOA) phenotypes, we noted the frequency of CD4+ FoxP3+ T cells was lower in individuals with SAR than with CSA. We also observed among the LOA patients, the frequency of CD4+ TGF-β+ T cells was decreased in SAR group than the in CSA group. Conclusion and clinical relevance Our data suggest that refractoriness to treatment in asthma is associated with a lower expression of distinct regulatory molecules by CD4 T cells between those who are atopic and have EOA and those who are non-atopic and have LOA. Thus, these results may contribute to the identification of new regulatory strategies to treat asthma according to their phenotypes. |
| Databáze: | OpenAIRE |
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