The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L
| Autor: | Jörg Kobarg, C. T. Santos, Leandro H.P. Assis, Daniel M. Trindade, Roy E. Larson, Rodrigo V. Honorato, Rui M.P. da Silva-Júnior, Priscila Oliveira de Giuseppe, Enilza Maria Espreáfico, Talita Diniz Melo-Hanchuk, Mário T. Murakami, Marcos Rodrigo Alborghetti, Paulo S. Oliveira, Luciano G. Dolce, Celisa Caldana Costa Tonoli, A.F.Z. Nascimento |
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| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine Protein Conformation Myosin Type V Plasma protein binding Biology Article Conserved sequence 03 medical and health sciences Protein structure Myosin Molecular motor Humans Protein Interaction Domains and Motifs Amino Acid Sequence Cilia Conserved Sequence Actin Adaptor Proteins Signal Transducing Centrosome Binding Sites Multidisciplinary Myosin Heavy Chains Cilium BIOLOGIA MOLECULAR Recombinant Proteins Cell biology 030104 developmental biology Biochemistry Protein Binding |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep43692 |
| Popis: | Myosin Va (MyoVa) is an actin-based molecular motor abundantly found at the centrosome. However, the role of MyoVa at this organelle has been elusive due to the lack of evidence on interacting partners or functional data. Herein, we combined yeast two-hybrid screen, biochemical studies and cellular assays to demonstrate that MyoVa interacts with RPGRIP1L, a cilia-centrosomal protein that controls ciliary signaling and positioning. MyoVa binds to the C2 domains of RPGRIP1L via residues located near or in the Rab11a-binding site, a conserved site in the globular tail domain (GTD) from class V myosins. According to proximity ligation assays, MyoVa and RPGRIP1L can interact near the cilium base in ciliated RPE cells. Furthermore, we showed that RPE cells expressing dominant-negative constructs of MyoVa are mostly unciliated, providing the first experimental evidence about a possible link between this molecular motor and cilia-related processes. |
| Databáze: | OpenAIRE |
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