In vitro study on the effect of doxycycline on the microbial activity of soil determined by redox-potential measuring system
| Autor: | Istvan Szatmari, Katalin Szakmár, Noémi László, Péter Laczay, Orsolya Erdősi, Zsuzsanna Szili, Péter Székely Körmöczy, Olivér Reichart |
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| Rok vydání: | 2014 |
| Předmět: |
Doxycycline
General Immunology and Microbiology Soil test Bacteria Colony Count Microbial Biological activity Soil classification General Medicine Biology Soil type Redox Chemistry Techniques Analytical Microbiology Anti-Bacterial Agents Soil Viable count medicine Potency Soil Pollutants Food science Oxidation-Reduction Soil Microbiology medicine.drug |
| Zdroj: | Acta microbiologica et immunologica Hungarica. 61(3) |
| ISSN: | 1217-8950 |
| Popis: | The potential effect of doxycycline on the microbial activity was investigated in three types of soil. Soil samples were spiked with doxycycline, incubated at 25°C and tested at 0, 2, 4 and 6 days after treatment. The microbiological activity of the soil was characterized by the viable count determined by plate pouring and by the time necessary to reach a defined rate of the redox-potential decrease termed as time to detection (TTD).The viable count of the samples was not changed during the storage. The TTD values, however exhibited a significant increase in the 0.2–1.6 mg/kg doxycycline concentration range compared to the untreated samples indicating concentration-dependent inhibitory effect on microbial activity. The potency of the effect was different in the 3 soil types. To describe the combined effect of the doxycycline concentration and time on the biological activity of one type of soil a mathematical model was constructed and applied.The change of microbial metabolic rate could be measured also without (detectable) change of microbial count when the traditional microbiological methods are not applicable. The applied new redox potential measurement-based method is a simple and useful procedure for the examination of microbial activity of soil and its potential inhibition by antibiotics. |
| Databáze: | OpenAIRE |
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