The wheat TdRL1 is the functional homolog of the rice RSS1 and promotes plant salt stress tolerance

Autor: Shin Takeda, Yutaka Tamari, Etienne Herzog, Chantal Ebel, Habib Mahjoubi, Marie-Edith Chabouté, Oumaya Bouchabke-Coussa, Moez Hanin, Anne-Catherine Schmit
Přispěvatelé: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Protection et Amélioration des Plantes, Centre de Biotechnologie de Sfax (CBS), Aix-Marseille Université - Faculté de médecine (AMU MED), Aix Marseille Université (AMU), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Chabouté, Marie-Edith, Université de Strasbourg (UNISTRA), Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Leupeptins
Arabidopsis
Plant Science
Sodium Chloride
01 natural sciences
Plant Roots
Gene Expression Regulation
Plant
Cellular localization
ComputingMilieux_MISCELLANEOUS
Triticum
Plant Proteins
2. Zero hunger
biology
Protein Stability
Cell Cycle
General Medicine
Salt Tolerance
Plants
Genetically Modified
Cell biology
RSS1
Phenotype
Seeds
Transgene
Salt
Green Fluorescent Proteins
Germination
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Genes
Plant
03 medical and health sciences
Stress
Physiological
Mitosis
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Durum wheat TdRL1
Sequence Homology
Amino Acid
Abiotic stress
Genetic Complementation Test
Wild type
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Oryza
Meristem
biology.organism_classification
Oxidative Stress
030104 developmental biology
Gene Ontology
Cytoplasm
Mutation
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Plant Cell Reports
Plant Cell Reports, Springer Verlag, 2018, 37 (12), pp.1625-1637. ⟨10.1007/s00299-018-2333-2⟩
Plant Cell Reports, 2018, 37 (12), pp.1625-1637. ⟨10.1007/s00299-018-2333-2⟩
Plant Cell Reports, Springer Verlag, 2018
ISSN: 1432-203X
0721-7714
DOI: 10.1007/s00299-018-2333-2⟩
Popis: International audience; Key messageRice rss1 complementation assays show that wheat TdRL1 and RSS1 are true functional homologs. TdRL1 over-expression in Arabidopsis conferred salt stress tolerance and alleviated ROS accumulation.AbstractPlants have developed highly flexible adaptive responses to their ever-changing environment, which are often mediated by intrinsically disordered proteins (IDP). RICE SALT SENSITIVE 1 and Triticum durum RSS1-Like 1 protein (TdRL1) are both IDPs involved in abiotic stress responses, and possess conserved D and DEN-Boxes known to be required for post-translational degradation by the APC/C-cdc20 cyclosome. To further understand their function, we performed a computational analysis to compare RSS1 and TdRL1 co-expression networks revealing common gene ontologies, among which those related to cell cycle progression and regulation of microtubule (MT) networks were over-represented. When over-expressed in Arabidopsis, TdRL1::GFP was present in dividing cells and more visible in cortical and endodermal cells of the Root Apical Meristem (RAM). Incubation with the proteasome inhibitor MG132 stabilized TdRL1::GFP expression in RAM cells showing a post-translational regulation. Moreover, immuno-cytochemical analyses of transgenic roots showed that TdRL1 was present in the cytoplasm and within the microtubular spindle of mitotic cells, while, in interphasic cells, it was rather restricted to the cytoplasm with a spotty pattern at the nuclear periphery. Interestingly in cells subjected to stress, TdRL1 was partly relocated into the nucleus. Moreover, TdRL1 transgenic lines showed increased germination rates under salt stress conditions as compared to wild type. This enhanced salt stress tolerance was associated to an alleviation of oxidative damage. Finally, when expressed in the rice rss1 mutant, TdRL1 suppressed its dwarf phenotype upon salt stress, confirming that both proteins are true functional homologs required for salt stress tolerance in cereals.
Databáze: OpenAIRE
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