Nuclear localization of Munc18-1 (p67) in the adult rat brain and PC12 cells
| Autor: | Rita Christopher, Taranath K. Shetty, Y. Ramamohan, Pavankumar A. Shetty, Momin M. Shareef, Savitha Kalidas, Vandana M. Sharma, Harish C. Pant, Kiran Bhaskar |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cytoplasm Vesicle docking Immunoelectron microscopy Nuclear Localization Signals Active Transport Cell Nucleus Vesicular Transport Proteins Fluorescent Antibody Technique Nerve Tissue Proteins Biology PC12 Cells Rats Sprague-Dawley Cellular and Molecular Neuroscience Munc18 Proteins Microscopy Electron Transmission medicine Animals Cytoskeleton Gene knockout Cell Nucleus Neurons Brain Cyclin-Dependent Kinase 5 DNA Cell Biology DNA-binding domain Immunohistochemistry Molecular biology Cyclin-Dependent Kinases Cell Compartmentation Protein Structure Tertiary Rats Cell biology DNA-Binding Proteins medicine.anatomical_structure Neuron Nuclear transport Nuclear localization sequence |
| Zdroj: | Neurochemistry International. 47:225-234 |
| ISSN: | 0197-0186 |
| DOI: | 10.1016/j.neuint.2005.01.005 |
| Popis: | Munc18-1, also referred to as p67, co-purifies with Cdk5 and has an important role in neurotransmitter release. The role of Munc18-1 for functional connectivity of the nervous system was demonstrated by gene knockout experiments in mice, wherein accumulation of neurotransmitter and silencing of synaptic activity was observed. Our earlier studies have shown that both Munc18-1 and Cdk5 co-purify and co-localize with cytoskeletal components, implying that apart from having a regulatory role in vesicle docking and fusion, Munc18-1 could also affect the dynamics of neuronal cytoskeleton. In the present study we have shown the presence of Munc18-1 in nuclear rich fraction from rat brain and confirmed the nuclear localization of this protein in PC12 cells and adult rat brain neurons by immunofluorescence and immunoelectron microscopy. We also demonstrate the binding of Munc18-1 to double stranded (ds) DNA. The ability of Munc18-1 to bind dsDNA, albeit the lack of DNA binding domains, suggests that the binding may be mediated through protein-protein interaction through some other DNA-binding proteins. The presence of both nuclear import and export signals in Munc18-1 primary structure corroborates its nuclear localization and makes it a putative shuttle protein between nuclear and cytoplasmic compartments, the precise physiological relevance of which needs to be elucidated. |
| Databáze: | OpenAIRE |
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