MTHFR C677T polymorphism in chronic pancreatitis and pancreatic adenocarcinoma
Autor: | Marina Andjelic-Jelic, Milenko Ugljesic, Ivan Nisevic, Natasa Petrovic-Stanojevic, Dragica Radojkovic, Valentina Djordjevic, Jelena Dinić, Aleksandra Nikolic, Snezana Lukic |
---|---|
Rok vydání: | 2008 |
Předmět: |
Adult
medicine.medical_specialty Pathology Genotype Clinical Biochemistry Population Adenocarcinoma Reductase Polymorphism Single Nucleotide Biochemistry Gastroenterology Gene Frequency Risk Factors Internal medicine Odds Ratio medicine Humans education Prospective cohort study Allele frequency Methylenetetrahydrofolate Reductase (NADPH2) Aged education.field_of_study biology business.industry Cell Biology General Medicine Middle Aged medicine.disease digestive system diseases Pancreatic Neoplasms Pancreatitis Methylenetetrahydrofolate reductase Chronic Disease biology.protein Gene polymorphism business |
Zdroj: | Cell Biochemistry and Function. 26:659-663 |
ISSN: | 1099-0844 0263-6484 |
Popis: | Chronic pancreatitis and pancreatic adenocarcinoma are extensively studied as common and potentially lethal disorders. However, their causes and genetic background in most cases remain unclear. The C677T polymorphism in 5′,10′-methylenetetrahydrofolate reductase (MTHFR) gene may modulate the risk of pancreatic disorders. In this study, we tested whether MTHFR C677T polymorphism is associated with chronic pancreatitis and pancreatic adenocarcinoma in the Serbian population. DNA was extracted from blood samples of 51 chronic pancreatitis patients, 21 pancreatic adenocarcinoma patients, and a control group consisting of 50 healthy smokers. The MTHFR C677T polymorphism was analyzed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique. Although, no statistically significant differences were observed in the distribution of MTHFR genotype or allele frequencies between patients and control groups, the results showed an increased frequency of homozygotes for MTHFR C677T polymorphism in chronic pancreatitis patients (14%) and a decreased frequency in pancreatic adenocarcinoma patients (5%) in comparison to the control group (8%). We speculate that the MTHFR C677T polymorphism could act as a possible risk factor for chronic pancreatitis and a possible protective factor in pancreatic adenocarcinoma. This observation needs further investigation in prospective studies on a larger number of patients, in which the effect of other genetic and environmental factors should also be taken into consideration. Copyright © 2008 John Wiley & Sons, Ltd. |
Databáze: | OpenAIRE |
Externí odkaz: |