Skeletal Myoblast Cell Sheet Implantation Ameliorates Both Systolic and Diastolic Cardiac Performance in Canine Dilated Cardiomyopathy Model
| Autor: | Yutaka Okita, Tomonori Shirasaka, Shigeru Miyagawa, Takashi Daimon, Satoshi Nakatani, Yoshiki Sawa, Satsuki Fukushima, Naomasa Kawaguchi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cardiac function curve
Cardiomyopathy Dilated medicine.medical_specialty Time Factors Systole medicine.medical_treatment Myoblasts Skeletal Diastole Cardiomyopathy Cell Culture Techniques 030204 cardiovascular system & hematology Collagen Type I Ventricular Function Left 03 medical and health sciences 0302 clinical medicine Dogs Original Basic Science—General Internal medicine medicine Animals Ventricular remodeling Collagen Type II Cells Cultured Transplantation Ejection fraction Ventricular Remodeling business.industry Myocardium Cardiac Pacing Artificial Stroke Volume Recovery of Function medicine.disease Fibrosis Disease Models Animal 030220 oncology & carcinogenesis Ventricular assist device Heart failure Cardiology Female business |
| Zdroj: | Transplantation |
| ISSN: | 1534-6080 |
| Popis: | Background Improving both systolic and diastolic function may be the most important factor in treating heart failure. In this study, we hypothesized that cell-sheet transplantation could improve these function in the damaged heart. Methods We generated a dilated cardiomyopathy model in beagles by continuous ventricle pacing at 240 beats per minute. After 4 weeks, the beagles underwent skeletal myoblast cell sheet transplantation (SMCST) or a sham operation, and rapid ventricle pacing continued for an additional 4 weeks. Six of the e8 beagles treated by SMCST were still alive 4 weeks after the procedure. We evaluated SMCST's cardiotherapeutic effects by comparing beagles treated by SMCST with beagles that underwent a sham operation (control, n = 5). Results Diastolic function, as well as systolic function improved significantly in the SMCST group as compared with the sham group (control vs SMCST group, median [interquartile range]: E/E', 16 [0.9] vs 11 [1.0]; P < 0.001; tau, 47 [6.0] vs 36 [4.4] ms: P = 0.005. Ejection fraction, 22 (6.0) versus 46 (7.5) %, P < 0.001; end-systolic elastance, 2.5 (0.4) versus 8.2 (3.5) mm Hg/ml, P = 0.001). Histological examination revealed that the volume of collagen I and the collagen I/III ratio in the myocardium were significantly higher in the control than that in the SMCST group (collagen I, 6.0 [0.8] vs 2.6 [1.3]; P = 0.006; collagen I/III ratio, 4.8 [1.7] vs 1.2 [0.4]; P = 0.010). Conclusions The potential of SMCST to ameliorate both systolic and diastolic performance was proven. The SMCST may be an alternative therapy of conventional medical treatment in the dilated cardiomyopathy heart. |
| Databáze: | OpenAIRE |
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