There is detectable variation in the lipidomic profile between stable and progressive patients with idiopathic pulmonary fibrosis (IPF)
| Autor: | Nicole S L Goh, Yuben Moodley, Britt Clynick, Dino Tan, Adam King, Shabarinath Nambiar, Robert D. Trengove, Tamera J. Corte, Bong S How, E. Haydn Walters |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty 01 natural sciences Mass Spectrometry Pathogenesis 03 medical and health sciences Idiopathic pulmonary fibrosis Plasma Fibrosis DIA Medicine Humans Registries SONAR Asthma Aged lcsh:RC705-779 COPD Lung business.industry Research 010401 analytical chemistry Lipid metabolism MS lcsh:Diseases of the respiratory system respiratory system medicine.disease Lipid Metabolism Lipids Idiopathic Pulmonary Fibrosis 0104 chemical sciences respiratory tract diseases 030104 developmental biology medicine.anatomical_structure IPF Lipidomics Disease Progression Female business Progressive disease Chromatography Liquid Follow-Up Studies |
| Zdroj: | Respiratory Research, Vol 22, Iss 1, Pp 1-8 (2021) Respiratory Research |
| Popis: | Background Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial lung disease characterized by fibrosis and progressive loss of lung function. The pathophysiological pathways involved in IPF are not well understood. Abnormal lipid metabolism has been described in various other chronic lung diseases including asthma and chronic obstructive pulmonary disease (COPD). However, its potential role in IPF pathogenesis remains unclear. Methods In this study, we used ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) to characterize lipid changes in plasma derived from IPF patients with stable and progressive disease. We further applied a data-independent acquisition (DIA) technique called SONAR, to improve the specificity of lipid identification. Results Statistical modelling showed variable discrimination between the stable and progressive subjects, revealing differences in the detection of triglycerides (TG) and phosphatidylcholines (PC) between progressors and stable IPF groups, which was further confirmed by mass spectrometry imaging (MSI) in IPF tissue. Conclusion This is the first study to characterise lipid metabolism between stable and progressive IPF, with results suggesting disparities in the circulating lipidome with disease progression. |
| Databáze: | OpenAIRE |
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