Ameliorative Effect of Ascorbic Acid and Glutathione in Combating the Cryoinjuries During Cryopreservation of Exotic Jack Semen
| Autor: | Jitendra Singh Mehta, T. R. Talluri, Rabindra Kumar, B.N. Tripathi, Prashant Kumar, S. C. Mehta, Mohd. Matin Ansari, Sanjay Kumar Ravi, Ashok Kumar Chaudhary, R. A. Legha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system 040301 veterinary sciences Semen Ascorbic Acid urologic and male genital diseases Semen collection Cryopreservation law.invention 0403 veterinary science Andrology chemistry.chemical_compound fluids and secretions law Animals Horses Dna integrity urogenital system Equine Extender 0402 animal and dairy science 04 agricultural and veterinary sciences Glutathione Ascorbic acid 040201 dairy & animal science Semen Analysis chemistry Female Cryoprotective Effect |
| Zdroj: | Journal of Equine Veterinary Science. 81:102796 |
| ISSN: | 0737-0806 |
| Popis: | The present study was designed to study the adverse effects of cryopreservation and evaluation of the cryoprotective effect of reduced glutathione (GSH) and ascorbic acid (AA) supplementation on exotic jack semen in combination or alone. For this, 24 semen samples from four adult and fertile jacks were collected via artificial vagina using an estrus jenny as dummy. After semen collection, the semen was evaluated for various qualitative and quantitative parameters in fresh, cooled, and frozen-thawed semen. The semen pellet was extended with the freezing extender containing either AA (0.9 g/L), GSH (2.5 mM), or combination of both (AA 0.9 g/L + GSH 2.5 mM), and another aliquot was kept as control without adding the antioxidants. The jack semen underwent cryodamage, which was evident by the observation of significant (P.05) decline in the seminal quantitative parameters at various stages of cryopreservation process. Prefreeze and postthaw semen evaluation revealed that the values of plasma membrane, acrosome integrity, and chromatin integrity were found to be significantly higher (P.01) in the group of samples supplemented with the combination (0.9 g/L AA +2.5 mM GSH) than AA- and GSH-alone or control groups. Supplementation of antioxidants to the freezing extender improved jack prefreeze and postthaw semen quality with the superiority of GSH over AA alone. From the present study, it was inferred that, exotic jack spermatozoa are susceptible to injuries because of cryopreservation, but these cryo-induced damage can be ameliorated significantly (P.05) with the use of antioxidants and contribute to the improvement of semen cryopreservation procedures. |
| Databáze: | OpenAIRE |
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