Transcriptomic analysis of Lycium ruthenicum Murr. during fruit ripening provides insight into structural and regulatory genes in the anthocyanin biosynthetic pathway

Autor: Feng Zhang, Fu-Ping Tian, Chun-Mei Wang, Yi Li, Hongshan Yang, Huirong Duan, Rui Ma, Yanjun Ma, Xue-Hui Zhou
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Lycium ruthenicum
Gene Expression
01 natural sciences
Biochemistry
Transcriptome
Anthocyanins
Database and Informatics Methods
MYB
Regulator gene
Genetics
Multidisciplinary
biology
Transcriptional Control
Structural gene
food and beverages
Eukaryota
Ripening
Genomics
Plants
Lycium
Medicine
Transcriptome Analysis
Sequence Analysis
Research Article
Bioinformatics
Science
Sequence Databases
Biosynthesis
Research and Analysis Methods
Fruits
03 medical and health sciences
Gene Types
Gene Regulation
Illumina dye sequencing
Gene Expression Profiling
Organisms
Biology and Life Sciences
Computational Biology
Molecular Sequence Annotation
biology.organism_classification
Genome Analysis
WRKY protein domain
030104 developmental biology
Biological Databases
Structural Genes
Fruit
010606 plant biology & botany
Transcription Factors
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 12, p e0208627 (2018)
ISSN: 1932-6203
Popis: Fruit development in Lycium ruthenicum Murr. involves a succession of physiological and biochemical changes reflecting the transcriptional modulation of thousands of genes. Although recent studies have investigated the dynamic transcriptomic responses during fruit ripening in L. ruthenicum, most have been limited in scope, and thus systematic data representing the structural genes and transcription factors involved in anthocyanin biosynthesis are lacking. In this study, the transcriptomes of three ripening stages associated with anthocyanin accumulation, including S1 (green ripeness stage), S2 (skin color change) and S3 (complete ripeness stage) in L. ruthenicum were investigated using Illumina sequencing. Of a total of 43,573 assembled unigenes, 12,734 were differentially expressed during fruit ripening in L. ruthenicum. Twenty-five significantly differentially expressed structural genes (including PAL, C4H, 4CL, CHS, CHI, F3H, F3'H, F3'5'H, DFR, ANS and UFGT) were identified that might be associated with anthocyanin biosynthesis. Additionally, several transcription factors, including MYB, bHLH, WD40, NAC, WRKY, bZIP and MADS, were correlated with the structural genes, implying their important interaction with anthocyanin biosynthesis-related genes. Our findings provide insight into anthocyanin biosynthesis and regulation patterns in L. ruthenicum and offer a systematic basis for elucidating the molecular mechanisms governing anthocyanin biosynthesis in L. ruthenicum.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje