| Popis: |
Molecular recruitment into functional domains in the cell plasma membrane is an important prerequisite for many cellular processes, such as cell signaling, cell-matrix adhesion, or the formation of synapses. High precision measurements of the origin and evolution of these structures is key to their understanding ; however, experimental methods with which one can detect molecular dynamics and aggregation, with microsecond temporal resolution and at nanometer scales, are scarce.One of the most developed approaches, which is up to the task, is fluorescence correlation spectroscopy (FCS). In FCS, fluorescent particles diffuse through a laser excitation beam and emit pulses of light proportional to the particle brightness.One way to circumvent the issue of relating probe intensities to their number is to use inverse fluorescence correlation spectroscopy (iFCS). Here, as opposed to conventional FCS, the molecule of interest is not labeled but diffuses in a homogeneously fluorescent environment, giving rise to negative stochastic intensity fluctuations. |
