Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk
Autor: | S. Del Vedovo, J. Ruiz, H. Villafan, Jean-Richard Neeser, A. Tello, A. Cravioto |
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Rok vydání: | 1991 |
Předmět: |
Immunodiffusion
Molecular Sequence Data Oligosaccharides Breast milk medicine.disease_cause digestive system Bacterial Adhesion Microbiology Cell Line Cohort Studies fluids and secretions Lactation medicine Escherichia coli Immunology and Allergy Humans Enteropathogenic Escherichia coli chemistry.chemical_classification biology Milk Human Colostrum food and beverages biochemical phenomena metabolism and nutrition Oligosaccharide biology.organism_classification Enterobacteriaceae Infectious Diseases medicine.anatomical_structure chemistry Carbohydrate Sequence Immunoglobulin A Secretory biology.protein bacteria Female Antibody Bacterial Outer Membrane Proteins |
Zdroj: | The Journal of infectious diseases. 163(6) |
ISSN: | 0022-1899 |
Popis: | Secretory IgA (sIgA) purified from colostrum and breast milk obtained from 14 women inhibited the localized adherence of an enteropathogenic Escherichia coli (EPEC) to HEp-2 cells. Inhibition decreased as lactation continued even when the concentration of sIgA was maintained constant at 1 mg/ml. sIgA responded to a 94-kDa plasmid-encoded outer membrane protein implicated as the EPEC adherence factor. An oligosaccharide-enriched fraction (OEF) from these samples also inhibited the attachment of this EPEC. Inhibition by OEFs decreased as lactation continued because of a general reduction in oligosaccharide content. Localized adherence of six other EPEC was also inhibited by sIgA and OEF, whereas attachment of isolates with diffuse or aggregative adherence was not inhibited by these fractions. Experiments with purified oligosaccharide fractions revealed that EPEC attach to HEp-2 cells through a carbohydrate-mediated mechanism based on the preferential recognition of fucosylated residues in human milk. |
Databáze: | OpenAIRE |
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