Endogenous IL-7 is associated with increased thymic volume in adult HIV-infected patients under highly active antiretroviral therapy
| Autor: | Rafael de la Rosa, Amalia Rubio, Manuel Leal, Natalia Soriano, Jaime M. Franco, Armando Sánchez-Quijano, Ezequiel Ruiz-Mateos, Eduardo Lissen, Manuel Martinez-Moya |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male T cell medicine.medical_treatment Immunology Receptors Antigen T-Cell HIV Infections Thymus Gland Polymerase Chain Reaction Flow cytometry Immune system Antigen Antiretroviral Therapy Highly Active Immunopathology medicine Humans Immunology and Allergy Chemotherapy medicine.diagnostic_test business.industry Interleukin-7 Flow Cytometry CD4 Lymphocyte Count Infectious Diseases Real-time polymerase chain reaction medicine.anatomical_structure Leukocytes Mononuclear RNA Viral Female Viral disease Tomography X-Ray Computed business Immunologic Memory Follow-Up Studies |
| Zdroj: | AIDS. 17:947-954 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/00002030-200305020-00002 |
| Popis: | Objective Immune reconstitution after highly active antiretroviral therapy (HAART) in HIV-infected patients has led to an increase in the number of new CD4 T lymphocytes. Neolymphopoiesis in the thymus has been proposed as a mechanism in T-cell regeneration. Nevertheless, factors involved in the regeneration of T cells by thymic-dependent pathways in HIV-infected patients under HAART are still unknown and might be of relevance in HIV infection. The aim of this work was to study the role of IL-7 in the thymic rebound of HIV-infected adults under HAART. Design To study the association between IL-7 and thymic function-related markers, these variables were measured in 49 antiretroviral-naive HIV-infected patients at baseline and at weeks 12, 24, 36 and 48 of treatment. Methods Thymic function-related markers: thymic volume, naive phenotype, and T-cell receptor excision circles (TREC) bearing-cells, were evaluated by computed tomography, flow cytometry, and quantitative polymerase chain reaction, respectively. IL-7 levels were evaluated using a high sensitivity colorimetric enzyme-linked immunosorbent assay. Results At baseline, we found an inverse correlation between IL-7 levels and thymic function-associated parameters: thymic volume, naive T cells and TREC-bearing cells. After 48 weeks of therapy increased levels of thymic function-related markers along with a significant decrease in IL-7 levels were found. IL-7 levels at baseline were the only independently associated variable with respect to changes in thymic volume at weeks 12, 24 and 48 of follow-up. Conclusion These data suggest that IL-7 plays an important role in thymic rebound in adult HIV-infected patients under HAART. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|