Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food
Autor: | Poh Fong Chuah, Carine Blancpain, Geoffrey Cottenet, Véronique Sonnard |
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Rok vydání: | 2019 |
Předmět: |
Food
Genetically Modified 010401 analytical chemistry Fast pcr 04 agricultural and veterinary sciences General Medicine Proficiency test Computational biology Biology Real-Time Polymerase Chain Reaction Absolute limit 040401 food science 01 natural sciences 0104 chemical sciences Analytical Chemistry Genetically modified organism 0404 agricultural biotechnology Real-time polymerase chain reaction Screening method Multiplex Multiplex Polymerase Chain Reaction Food Science |
Zdroj: | Food Chemistry. 274:760-765 |
ISSN: | 0308-8146 |
Popis: | Methods to detect the presence of genetically modified organisms (GMOs) are evolving constantly to comply with legislation and include new transgenic traits developed by biotechnology companies. Since traditional screening methods target only a limited number of markers, not all GM-events can be detected easily. To cover a broader range, a new GMO screening method was developed, based on two real-time PCR reactions, targeting i) six major GM-markers and ii) six GM-events that do not contain these markers. This method used the FAST PCR technology and allowed a further reduction in time-to-result. Using a wide array of reference materials and proficiency test samples, the method showed a broad screening capacity and the absolute limit of detection was consistently below 20 copies, thereby demonstrating the method is fit-for-purpose. |
Databáze: | OpenAIRE |
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