NR5A2 and potential regulatory miRNAs in the bovine CL during early pregnancy
Autor: | Camilla K. Hughes, E. K. Inskeep, Joy L. Pate, A Rogus |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
endocrine system Embryology Prostaglandin Receptors Cytoplasmic and Nuclear Estrous Cycle Biology Luteal phase Dinoprost Andrology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Corpus Luteum Pregnancy medicine Animals Progesterone Estrous cycle 030219 obstetrics & reproductive medicine Liver receptor homolog-1 Obstetrics and Gynecology Cell Biology Interferon tau MicroRNAs 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Nuclear receptor chemistry HSD3B1 Cattle Female Corpus luteum |
Zdroj: | Reproduction (Cambridge, England). 161(2) |
ISSN: | 1741-7899 |
Popis: | Progesterone, which is secreted from the corpus luteum, is indispensable for the establishment and maintenance of pregnancy. The orphan nuclear receptor subfamily 5 group A member 2 (NR5A2) is a regulator of murine luteinization, but neither its regulation nor its role in the fully differentiated, mature corpus luteum (CL) have been described. Therefore, the goal of this study was to profile abundance and investigate the regulation and functions of NR5A2 in the bovine CL. Treatment of cultured luteal steroidogenic cells with a pharmacological inhibitor of NR5A2 decreased progesterone production and tended to decrease abundance of HSD3B1 mRNA. Luteal NR5A2 mRNA increased and NR5A2 protein tended to increase between days 4 and 6 of the estrous cycle, coincident with increased steroidogenic capacity of the CL. Luteal NR5A2 mRNA decreased by 8 h after prostaglandin (PG) F2A injection. During early pregnancy, luteal NR5A2 mRNA was less on days 20 and 23 compared to day 14, but protein abundance did not change. Neither 1 nor 10 ng/mL interferon tau (IFNT) altered NR5A2 abundance in cultured luteal steroidogenic cells, but 10 ng/mL PGF2A decreased NR5A2. Because of discrepancies between mRNA and protein abundance of NR5A2, regulation by miRNA that changed during early pregnancy was investigated. miR-27b-3p, miR-432-5p, and miR-369-3p mimics decreased NR5A2 protein abundance and miR-369-3p also inhibited progesterone production. Overall, the results of this study show that NR5A2 may be maintained by miRNA during early pregnancy and may be an important regulator of luteal progesterone production. |
Databáze: | OpenAIRE |
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