Distribution of zinc metallothionein I mRNA in rat brain using in situ hybridization
| Autor: | Ronald F. Pfeiffer, J. F. Rodriguez-Sierra, H. S. Blaxall, R. Hao, D. R. Cerutis, Manuchair Ebadi |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Gene isoform DNA Complementary Central nervous system In situ hybridization Hippocampal formation Biology Sulfur Radioisotopes Biochemistry Rats Sprague-Dawley Cellular and Molecular Neuroscience Complementary DNA medicine Animals Metallothionein RNA Messenger Northern blot In Situ Hybridization Messenger RNA Brain General Medicine Blotting Northern Molecular biology Rats Zinc medicine.anatomical_structure Liver nervous system Organ Specificity Autoradiography DNA Probes |
| Zdroj: | Neurochemical Research. 19:761-767 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/bf00967717 |
| Popis: | Metallothionein (MT) isoforms I and II were first identified and characterized in our laboratories in several regions of brain, in hippocampal neurons in primary culture, and in retinoblastoma and neuroblastoma cell lines. In this study, by having employed the MT-I cDNA as a probe, we sought to gain additional insight about the function of MT by discerning the regional distribution of its mRNA. Northern blot analyses of brain mRNA revealed that the administration of zinc enhanced dramatically MT-I mRNA (570 bp). The in situ hybridization study revealed that MT-I mRNA was located in several areas of brain, with the highest concentrations found in the cerebellum, hippocampus, and ventricles. The results of these studies are interpreted to suggest that zinc enhances the synthesis of MT mRNA and MT in turn may participate in zinc associated functions in neurons. |
| Databáze: | OpenAIRE |
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