Autologous platelet-poor plasma decreases the bronchial stump necrosis in rat
| Autor: | María D. Fiuza, Rafael Camacho, José A. Ruíz-Caballero, Yanira Brito-Godoy, Pedro Llontop, José Manuel García-Castellano, Norberto Santana-Rodríguez, Ardiel Quintana, Leandro Fernández-Pérez, Bernardino Clavo, Miguel A. Ponce-González, Keila Zerecero |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology medicine.medical_specialty animal structures Necrosis medicine.medical_treatment Bronchopleural fistula Gene Expression Bronchi Fibrous tissue digestive system Rats Sprague-Dawley Insulin-like growth factor Pneumonectomy Blood Transfusion Autologous Plasma Autologous plasma medicine Animals Autologous platelet Insulin-Like Growth Factor I Wound Healing business.industry Bronchial stump respiratory system Pleural Diseases medicine.disease Rats body regions surgical procedures operative Surgery Bronchial Fistula medicine.symptom business |
| Zdroj: | The Journal of surgical research. 183(1) |
| ISSN: | 1095-8673 |
| Popis: | Background Necrosis of the bronchial stump is a very important trigger for bronchopleural fistula. The administration of local autologous platelet-poor plasma (PPP) could protect the bronchial stump. Materials and methods Left pneumonectomy was performed in 25 Sprague-Dawley rats. Animals were randomly assigned to a control group ( n = 13) and PPP group ( n = 12). PPP was locally administered on the bronchial stump after pneumonectomy. We analyzed histologic changes in the bronchial stump and messenger RNA expression changes of genes involved in wound repair at 10 and 20 d. Results Local PPP treatment produced a mass of fibrous tissue surrounding the bronchial stump and significantly decreased the presence of necrosis at 20 d. PPP increased the expression of insulin like growth factor 1 at 10 d although it did not reach statistical significance. Conclusions Our findings indicate that local PPP treatment of the bronchial stump after pneumonectomy decreased necrosis and could have a protective effect on the bronchial stump. |
| Databáze: | OpenAIRE |
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