Synaptotagmin 5 Controls SYP132-VAMP721/722 Interaction for Arabidopsis Immunity to Pseudomonas syringae pv tomato DC3000
| Autor: | Keunchun Park, Hyeran Kim, Da Jeong Cho, Mi Kyung Kim, Soohong Kim, Chian Kwon, Hye Sup Yun |
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| Rok vydání: | 2021 |
| Předmět: |
Arabidopsis
Pseudomonas syringae Exocytosis Synaptotagmin 1 R-SNARE Proteins Synaptotagmins VAMP721/722 Plant defense against herbivory Molecular Biology Secretory pathway Plant Diseases biology Arabidopsis Proteins Qa-SNARE Proteins fungi food and beverages SYT5 Cell Biology General Medicine SYP132 biology.organism_classification Cell biology Membrane protein Erysiphe pisi Pst DC3000 plant immunity Research Article |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 |
| Popis: | Vesicle-associated membrane proteins 721 and 722 (VAMP721/722) are secretory vesicle-localized arginine-conserved soluble N-ethylmaleimide-sensitive factor attachment protein receptors (R-SNAREs) to drive exocytosis in plants. They are involved in diverse physiological processes in plants by interacting with distinct plasma membrane (PM) syntaxins. Here, we show that synaptotagmin 5 (SYT5) is involved in plant defense against Pseudomonas syringae pv tomato (Pst) DC3000 by regulating SYP132-VAMP721/722 interactions. Calcium-dependent stimulation of in vitro SYP132-VAMP722 interaction by SYT5 and reduced in vivo SYP132-VAMP721/722 interaction in syt5 plants suggest that SYT5 regulates the interaction between SYP132 and VAMP721/722. We interestingly found that disease resistance to Pst DC3000 bacterium but not to Erysiphe pisi fungus is compromised in syt5 plants. Since SYP132 plays an immune function to bacteria, elevated growth of surface-inoculated Pst DC3000 in VAMP721/722-deficient plants suggests that SYT5 contributes to plant immunity to Pst DC3000 by promoting the SYP132-VAMP721/722 immune secretory pathway. |
| Databáze: | OpenAIRE |
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