The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor
| Autor: | Philip N. Bartlett, Yann Astier |
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| Rok vydání: | 2004 |
| Předmět: |
Aniline Compounds
Time Factors Transistors Electronic Polymers Inorganic chemistry Biophysics Aminophenols Electrochemistry Sensitivity and Specificity Catalysis Glucose Oxidase Hydrolysis chemistry.chemical_compound Aniline Glucose oxidase Physical and Theoretical Chemistry biology Microchemistry Substrate (chemistry) General Medicine Buffer solution Alkaline Phosphatase Glucose chemistry Calibration biology.protein Alkaline phosphatase Gold Nuclear chemistry |
| Zdroj: | Bioelectrochemistry. 64:53-59 |
| ISSN: | 1567-5394 |
| Popis: | We report a new approach to the measurement of alkaline phosphatase concentration based on the use of a disposable poly(aniline) microelectrochemical transistor. The measurement is carried out in a two cell configuration in which the poly(aniline) microelectrochemical transistor operates in acid solution and is connected to the alkaline buffer solution containing the analyte by a salt bridge. Disposable microelectrochemical transistors were reproducibly fabricated by electrochemical deposition of poly(aniline) onto photolithographically fabricated gold microband arrays. Using these devices alkaline phosphatase was detected by employing p-aminophenyl phosphate as the substrate for the enzyme and using glucose and glucose oxidase to recycle the p-aminophenol generated upon enzyme catalysed hydrolysis of the phosphate. Recycling the p-aminophenol with glucose and glucose oxidase amplified the detection of alkaline phosphatase approximately tenfold. Using this approach we obtain linear calibration curves for alkaline phosphatase up to 5 nM within 70 s on single use devices. |
| Databáze: | OpenAIRE |
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