The development of immune responses in Balb/c mice following inoculation with attenuated or virulentNeospora caninumtachyzoites
| Autor: | Paul M. Bartley, Stephen E. Wright, Stephen W. Maley, Mintu Nath, E.A. Innes, D. Buxton |
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| Rok vydání: | 2009 |
| Předmět: |
Serum
Immunology Antibodies Protozoan Virulence Spleen Severity of Illness Index BALB/c Interferon-gamma Mice Neospora Immune system medicine Splenocyte Animals Mice Inbred BALB C biology Coccidiosis Body Weight Brain Histology biology.organism_classification Survival Analysis Neospora caninum medicine.anatomical_structure Leukocytes Mononuclear Female Parasitology |
| Zdroj: | Parasite Immunology. 31:392-401 |
| ISSN: | 1365-3024 0141-9838 |
| Popis: | Balb/c mice were inoculated intraperitoneally (i.p.) with either 5 x 10(6) live virulent (group 1) or 5 x 10(6) live attenuated (group 2) tachyzoites, or Vero cells (group 3). Animals were killed at 0, 14, 28 and 42 days post-inoculation (p.i.), with the remaining mice receiving a lethal challenge on day 48 p.i. Serum, spleen and brain samples were collected post-mortem to examine humoral and cell-mediated immune responses as well as pathological lesions and to quantify parasite loads. On day 14 p.i. group 2 (attenuated) demonstrated statistically significant (P < 0.001) lower levels of mean morbidity and weight loss, while also showing significantly (P = 0.01) higher levels of splenocyte proliferation and IFN-gamma production (P = 0.003), compared to group 1 (virulent). Histology of brain samples showed milder lesions and a lower incidence of positive immunohistochemistry, demonstrating tachyzoites and tissue cysts, and statistically significant (P = 0.03) lower mean burdens of parasite DNA in group 2 (attenuated) compared to group 1 (virulent). All mice in group 2 were protected following challenge on day 48 p.i. whereas naive control mice succumbed to the challenge. No mice from group 1 (virulent) survived beyond day 24 p.i. so they were not included in the challenge. |
| Databáze: | OpenAIRE |
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