Rejection quantity in kidney transplant recipients is associated with increasing intracellular interleukin-2 in CD8+ T-cells
| Autor: | Bora Akoglu, Said Emal Yosuf, Jan Goßmann, Heinz-Georg Kachel, D Faust, Stefan Zeuzem, Ernst-H. Scheuermann, Eva Herrmann, Barbara Lafferton, Shara Kalb |
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| Rok vydání: | 2014 |
| Předmět: |
Interleukin 2
Adult Graft Rejection Male Adolescent CD3 Immunology Intracellular Space CD8-Positive T-Lymphocytes Flow cytometry Young Adult medicine Immunology and Allergy Cytotoxic T cell Humans Risk factor Child Kidney transplantation Aged Aged 80 and over Transplantation medicine.diagnostic_test biology business.industry Middle Aged medicine.disease Flow Cytometry Kidney Transplantation Transplant Recipients biology.protein Interleukin-2 Female business CD8 Intracellular medicine.drug |
| Zdroj: | Transplant immunology. 31(1) |
| ISSN: | 1878-5492 |
| Popis: | CD8+ T-cells and interleukin-2 play an important role during organ rejection in kidney transplant recipients. Numerous studies showed increased interleukin-2 levels during acute rejection. The aim of our study is to show an association between intracellular interleukin-2 in CD8+ T-cells and the incidence of those who underwent organ rejection in kidney transplant recipients.407 transplant recipients were included into this study. The rejection incidence was investigated from the patient records. White blood cells from recipients were separated using a ficoll gradient. The cells were double-gated (CD3+ and CD8+) for the analysis of cellular percentage for intracellular interleukin-2 with a flow cytometer.The percentage of CD8+ cells with detectable intracellular interleukin-2 was significantly higher in renal transplant recipients with a documented rejection compared to recipients without any history of rejection (9.06±0.50, N=133 vs. 4.28±0.24, N=274, p0.0001, t-test). Further, there was a significant increase in patients with one (8.02±0.54, N=80, p0.0001, t-test), two (10.40±1.17, N=33, p0.0001, t-test) or three (11.82±1.58, N=18, p0.0001, t-test) rejection events.Past studies showed, that during acute organ rejection intracellular interleukin-2 is increased in cytotoxic T-cells, supposed to be a marker for this event. We were able to show, that intracellular interleukin-2 in CD8+ T-cells is also increased after organ rejection. Furthermore it seems to depend on the quantity of rejection events. Further studies in recipients with increased intracellular interleukin-2 in cytotoxic CD8+ T-cells and documented history of organ rejection are needed to identify this as a possible risk factor for further rejection events. |
| Databáze: | OpenAIRE |
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