Is Kaposi's-sarcoma-associated herpesvirus detectable in semen of HIV-infected homosexual men?
| Autor: | Jung-Chung Lin, Thomas J. Spira, Seh-Ching Lin, Eng-Chun Mar, John A. Stewart, Philip E. Pellett, Felicia R. Stamey |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
viruses Semen HIV Infections Biology medicine.disease_cause Polymerase Chain Reaction Virus Herpesviridae Acquired immunodeficiency syndrome (AIDS) HIV Seronegativity medicine Humans Kaposi's sarcoma-associated herpesvirus Homosexuality Male Sida Sarcoma Kaposi Retrospective Studies virus diseases General Medicine Sequence Analysis DNA biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Virology Immunology DNA Viral Viral disease Nested polymerase chain reaction |
| Zdroj: | Lancet (London, England). 346(8990) |
| ISSN: | 0140-6736 |
| Popis: | We explored a possible route of transmission of Kaposi's-sarcoma-associated herpes virus (KSHV) with nested and unnested PCR techniques. We looked for KSHV DNA sequences in semen of HIV-positive homosexual men and HIV-negative healthy semen donors. With unnested primers we found KSHV sequences in 21 of 33 (64%) homosexual men and in none of 30 healthy donors. With a nested PCR assay, 30 of 33 (91%) specimens from the homosexual men and 7 of 30 (23%) specimens from healthy donors had detectable KSHV sequences. Over 5 years of follow-up, 13 of 30 KSHV-positive homosexual men (43%) developed KS compared with none of the 3 KSHV-negative homosexual men. |
| Databáze: | OpenAIRE |
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