Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis:a preliminary investigation
| Autor: | T. W. Jones, M. V. Thrusfield, C. G. G. Aitken, P. F. Rae, A. G. Luckins, A. Higgins |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
endocrine system
Camelus Epidemiology Trypanosoma brucei brucei Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Antigens Protozoan Biology Serology Sudan Antigen Camels medicine Animals Serologic Tests Antiserum Trypanosoma evansi medicine.disease biology.organism_classification Virology Infectious Diseases Trypanosomiasis African biology.protein Antibody Protein A Trypanosomiasis Conjugate Research Article |
| Zdroj: | Rae, P F, Thrusfield, M V, Higgins, A, Aitken, C G, Jones, T W & Luckins, A G 1989, ' Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis : a preliminary investigation ', Epidemiology and Infection, vol. 102, no. 2, pp. 297-307 . |
| Popis: | SUMMARYThree enzyme immunoassays were used for the serodiagnosis of Trypanosoma evansi in camels in the Sudan in order to evaluate their ability to discriminate between infected and non-infected animals. Two assays were used for the detection of trypanosomal antibodies, one using specific anti-camel IgG conjugate and another using a non-specific Protein A conjugate. The third assay detected the presence of trypanosomal antigens using anti-T. evansi antibodies in a double antibody sandwich assay. Inspection of the frequency distribution of assay results suggested that the ELISA for circulating trypanosomal antibodies using specific antisera and the ELISA for circulating antigens can distinguish between non-infected camels and infected camels exhibiting patent infections or not. The ELISA using Protein A conjugate to bind non-specifically to camel immunoglobulin did not appear to discriminate between infected and non-infected animals. |
| Databáze: | OpenAIRE |
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