Functionalization of dynamic culture surfaces with a cartilage extracellular matrix extract enhances chondrocyte phenotype against dedifferentiation
| Autor: | Thomas M. Quinn, Sofia Solar-Cafaggi, Derek H. Rosenzweig |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Biomedical Engineering Fluorescent Antibody Technique Real-Time Polymerase Chain Reaction Biochemistry Chondrocyte Biomaterials Extracellular matrix Glycosaminoglycan chemistry.chemical_compound Silicone Gene expression medicine Animals Secretion Composite material Molecular Biology Cells Cultured Reverse Transcriptase Polymerase Chain Reaction Cartilage General Medicine Cell Dedifferentiation Extracellular Matrix Cell biology medicine.anatomical_structure chemistry Surface modification Cattle Biotechnology |
| Zdroj: | Acta Biomaterialia. 8:3333-3341 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2012.05.032 |
| Popis: | Culture on silicone rubber surfaces has been shown to partially overcome the chondrocyte dedifferentiation characteristic of standard culture on rigid polystyrene. These methods typically involve functionalization of culture surfaces with proteins. Collagen type I is often used, but more cartilage-specific proteins may be more appropriate for chondrocytes. To explore this hypothesis, a twofold experimental design was applied. First, chondrocytes were cultured in rigid Petri dishes coated with silicone rubber (“static silicone” or SS culture) functionalized with either cartilage extracellular matrix (ECM) extract or collagen type I. Second, chondrocytes were cultured on monotonically expanded high extension silicone rubber dishes (“continuous expansion” or CE culture) functionalized with ECM extract and compared to cells grown in SS culture. There were no differential effects of surface functionalization with the ECM extract vs. collagen type I on chondrocyte morphology, viability, proliferation or apoptosis in SS culture. However, chondrocyte growth on the ECM extract was associated with significantly reduced collagen types I and X gene expression and significantly increased glycosaminoglycan (GAG) secretion. After 3 passages (P3) on ECM-coated SS culture, chondrocyte phenotype and GAG secretion was enhanced compared to cells passaged on collagen type I. Pellet cultures from P3 SS culture displayed enhanced collagen type II content when ECM extract was used for functionalization rather than collagen type I. In CE culture with ECM functionalization, chondrocyte dedifferentiation was significantly inhibited vs. SS cultures, as evidenced by both gene expression and pellet cultures. Functionalization of extendable culture surfaces with cartilage ECM extract therefore supports enhanced preservation of chondrocyte phenotype. |
| Databáze: | OpenAIRE |
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