Antisense-induced ribosomal frameshifting
| Autor: | Christine B. Anderson, Michael T. Howard, Clark M. Henderson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Reticulocytes
viruses Molecular Sequence Data Reading frame Biology Ribosome Ribosomal frameshift Frameshift mutation 03 medical and health sciences Open Reading Frames Genetics Polyamines Animals Frameshift Mutation 030304 developmental biology 0303 health sciences Translational frameshift 030302 biochemistry & molecular biology Molecular Mimicry RNA Frameshifting Ribosomal Translation (biology) Oligonucleotides Antisense Cell biology Open reading frame Nucleic Acid Conformation Rabbits |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkl531 |
| Popis: | Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1–ORF2 fusion protein. Such programmed frameshifting is commonly utilized as a gene expression mechanism in viruses that infect eukaryotic cells and in a subset of cellular genes. RNA secondary structures, consisting of pseudoknots or stem–loops, located downstream of the shift site often act as cis-stimulators of frameshifting. Here, we demonstrate for the first time that antisense oligonucleotides can functionally mimic these RNA structures to induce +1 ribosomal frameshifting when annealed downstream of the frameshift site, UCC UGA. Antisense-induced shifting of the ribosome into the +1 reading frame is highly efficient in both rabbit reticulocyte lysate translation reactions and in cultured mammalian cells. The efficiency of antisense-induced frameshifting at this site is responsive to the sequence context 5′ of the shift site and to polyamine levels. |
| Databáze: | OpenAIRE |
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