Novel Fredericamycin Variant Overproduced by a Streptomycin-resistant Streptomyces albus subsp. chlorinus Strain
| Autor: | Andriy Luzhetskyy, Marta Rodríguez Estévez, Birgit Rosenkränzer, Lutz Petzke, Jeanette Ristau, Thomas Paululat, Maksym Myronovskyi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Mutant
Pharmaceutical Science 03 medical and health sciences Polyketide synthase Drug Discovery Gene cluster medicine overproduction Overproduction lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) fredericamycin Streptomyces albus 030304 developmental biology antitumor 0303 health sciences biology Strain (chemistry) 030306 microbiology secondary metabolites streptomycin-resistant biology.organism_classification lcsh:Biology (General) Biochemistry Streptomycin streptomycetes biology.protein type II PKS medicine.drug Biosynthetic genes |
| Zdroj: | Marine Drugs Volume 18 Issue 6 Marine Drugs, Vol 18, Iss 284, p 284 (2020) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md18060284 |
| Popis: | Streptomycetes are an important source of natural products potentially applicable in the pharmaceutical industry. Many of these drugs are secondary metabolites whose biosynthetic genes are very often poorly expressed under laboratory cultivation conditions. In many cases, antibiotic-resistant mutants exhibit increased production of natural drugs, which facilitates the identification and isolation of new substances. In this study, we report the induction of a type II polyketide synthase gene cluster in the marine strain Streptomyces albus subsp. chlorinus through the selection of streptomycin-resistant mutants, resulting in overproduction of the novel compound fredericamycin C2 (1). Fredericamycin C2 (1) is structurally related to the potent antitumor drug lead fredericamycin A. |
| Databáze: | OpenAIRE |
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