Visual Electrofluorochromic Detection of Cancer Cell Surface Glycoprotein on a Closed Bipolar Electrode Chip
| Autor: | Zhenxian Zhou, Fengying Shao, Yafeng Wu, Mingqiang Zou, Zhaoyan Tian, Li Mi, Songqin Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Aptamer
Biosensing Techniques Carbon nanotube 010402 general chemistry 01 natural sciences Analytical Chemistry law.invention chemistry.chemical_compound law Electrochemistry Humans Electrodes MUC1 Base Sequence Chemistry Mucin-1 010401 analytical chemistry Aptamers Nucleotide Combinatorial chemistry Fluorescence 0104 chemical sciences Anode Spectrometry Fluorescence Membrane Ferrocene Electrode MCF-7 Cells Oxidation-Reduction |
| Zdroj: | Analytical Chemistry. 91:7902-7910 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | This work reports an electrofluorochromic strategy on the basis of electric field control of fluorescent signal generation on bipolar electrodes (BPEs) for visualizing cancer cell surface glycoprotein (mucin 1). The device included two separate cells: anodic sensing cell and cathodic reporting cell, which were connected by a screen-printing electrode patterned on poly(ethylene terephthalate) (PET) membrane. In the sensing cell, anti-MUC1 antibody immobilized on a chitosan-multiwalled carbon nanotube (CS-MWCNT)-modified anodic BPE channel was used for capturing mucin-1 (MUC1) or MCF-7 cancer cells. Then ferrocene (Fc)-labeled mucin 1 aptamers were introduced through hybridization. Under an applied voltage, the ferrocene was oxidized and the electroactive molecules of 1,4-benzoquinone (BQ) in the cathodic reporting cell were reduced according to electroneutrality. This produced a strongly basic 1,4-benzoquinone anion radical (BQ•–), which turned on the fluorescence of pH-responsive fluorescent molecules of ... |
| Databáze: | OpenAIRE |
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