Naesohwangryeon-tang Induced Apoptosis and Autophagy in A549 Human Lung Cancer Cells
| Autor: | Cheol Park, Jin-Woo Jeong, Hong Jae Kim, Yung Hyun Choi, Su Hyun Hong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Apoptosis 03 medical and health sciences Naesohwangryeon-tang 0302 clinical medicine Autophagy MTT assay Viability assay lcsh:Miscellaneous systems and treatments Caspase Pharmacology chemistry.chemical_classification A549 cell Reactive oxygen species biology Cell growth lcsh:R lcsh:RM1-950 lcsh:RZ409.7-999 A549 Lung Cancer Cells lcsh:Therapeutics. Pharmacology 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Caspases biology.protein Cancer research Original Article Reactive Oxygen Species |
| Zdroj: | Journal of Pharmacopuncture Journal of Pharmacopuncture, Vol 22, Iss 4, Pp 269-278 (2019) |
| ISSN: | 2234-6856 2093-6966 |
| Popis: | Objectives Naesohwangryeon-tang (NHT) is a type of traditional herbal formula, however, little is known about its antitumor activity. In this study, the antitumor properties of NHT was evaluated in human lung adenocarcinoma cells. Methods To check the inhibitory effect of NHT, MTT assay was performed. Cell cycle analysis and detection of ROS production were conducted by flow cytometry. To evaluate the signaling pathway, Western blotting was conducted. Results Our results showed that the decrease of cell proliferation by NHT stimulation occurred more significantly in A549 cells than in NCI-H460 cells. In addition, NHT-induced apoptosis was associated with the activation of caspases and production of reactive oxygen species (ROS). NHT-induced apoptosis was attenuated after pretreatments with z-VAD-fmk or N-acetylcysteine, suggesting that NHT-induced apoptosis was caspase- and ROS-dependent. Interestingly, NHT treatment led to the development of autophagic vesicular organelles and upregulation of several autophagy-related genes. The pretreatment of bafilomycin A1 decreased apoptosis slightly but increased cell viability in the presence of NHT. Conclusion These findings indicated that NHT induces both apoptosis and cell-protective autophagy in human lung cancer cells. This data suggests that NHT might be a novel herbal drug for lung cancer. |
| Databáze: | OpenAIRE |
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