Homologous recombination changes the context of Cytochrome b transcription in the mitochondrial genome of Silene vulgaris KRA
| Autor: | Helena Štorchová, Daniel B. Sloan, Oushadee A. J. Abeyawardana, Jana Walterová, James D. Stone, Karel Müller, Marie Pazoutova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA Candidate gene Plant Infertility lcsh:QH426-470 lcsh:Biotechnology Chimeric gene Biology Genome Open Reading Frames 03 medical and health sciences lcsh:TP248.13-248.65 Gene expression Genetics Homologous Recombination Silene Gene Plant Proteins Membrane Glycoproteins Cytoplasmic male sterility Cytochromes b Mitochondrial Proton-Translocating ATPases Mitochondria lcsh:Genetics 030104 developmental biology Haplotypes RNA editing Essential gene Genome Mitochondrial RNA Editing Transcriptome Homologous recombination Research Article Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 19, Iss 1, Pp 1-17 (2018) |
| DOI: | 10.1101/422808 |
| Popis: | BackgroundSilene vulgaris (bladder campion) is a gynodioecious species existing as two genders – male-sterile females and hermaphrodites. Cytoplasmic male sterility (CMS) is generally encoded by mitochondrial genes, which interact with nuclear fertility restorer genes. Mitochondrial genomes of this species vary in DNA sequence, gene order and gene content. Multiple CMS genes are expected to exist in S. vulgaris, but little is known about their molecular identity.ResultsWe assembled the complete mitochondrial genome from the haplotype KRA of S. vulgaris. It consists of five chromosomes, two of which recombine with each other. Two small non-recombining chromosomes exist in linear, supercoiled and relaxed circle forms. We compared the mitochondrial transcriptomes from females and hermaphrodites and confirmed the differentially expressed chimeric gene bobt as the strongest CMS candidate gene in S. vulgaris KRA. The chimeric gene bobt is co-transcribed with the Cytochrome b (cob) gene in some genomic configurations. The co-transcription of a CMS factor with an essential gene may constrain transcription inhibition as a mechanism for fertility restoration because of the need to maintain appropriate production of the necessary protein. Homologous recombination places the gene cob outside the control of bobt, which allows for the suppression the CMS gene by the fertility restorer genes. In addition, by analyzing RNA editing, we found the loss of three editing sites in the KRA mitochondrial genome and identified four sites with highly distinct editing rates between KRA and another S. vulgaris haplotypes (KOV). Three of these highly differentially edited sites were located in the transport membrane protein B (mttB) gene. They resulted in differences in MttB protein sequences between haplotypes despite completely identical gene sequences.ConclusionsFrequent homologous recombination events that are widespread in plant mitochondrial genomes may change chromosomal configurations and also the control of gene transcription including CMS gene expression. Posttranscriptional processes, e.g RNA editing shall be evaluated in evolutionary and co-evolutionary studies of mitochondrial genes, because they may change protein composition despite the sequence identity of the respective genes. The investigation of natural populations of wild species such as S. vulgaris are necessary to reveal important aspects of CMS missed in domesticated crops, the traditional focus of the CMS studies. |
| Databáze: | OpenAIRE |
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