Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor
| Autor: | Rakesh Lodha, Soon Jyoti Das, Patil Sharanabasava, Guruprasad R. Medigeshi, Sushil K. Kabra, Tarun Kumar Sharma, Anbalagan Anantharaj |
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| Rok vydání: | 2020 |
| Předmět: |
DNAzyme
Computer science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Deoxyribozyme 02 engineering and technology Computational biology 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry law.invention real-time-PCR sensor law Molecular Biosciences lcsh:QH301-705.5 colorimetric assay Molecular Biology Polymerase chain reaction Thermal cycler SARS-CoV-2 010401 analytical chemistry COVID-19 RNA Gold standard (test) Brief Research Report 021001 nanoscience & nanotechnology 0104 chemical sciences Visual detection Real-time polymerase chain reaction lcsh:Biology (General) 0210 nano-technology |
| Zdroj: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Vol 7 (2020) |
| ISSN: | 2296-889X |
| DOI: | 10.3389/fmolb.2020.586254 |
| Popis: | The gold standard for the diagnosis of SARS-CoV-2, the causative agent of COVID-19, is real-time polymerase chain reaction (PCR), which is labor-intensive, expensive, and not widely available in resource-poor settings. Therefore, it is imperative to develop novel, accurate, affordable, and easily accessible assays/sensors to diagnose and isolate COVID-19 cases. To address this unmet need, we utilized the catalytic potential of peroxidase-like DNAzyme and developed a simple visual detection assay for SARS-CoV-2 RNA using a conventional thermal cycler by the PCR-induced generation of DNAzyme sensor. The performance of RT-PCR DNAzyme-based sensor was comparable to that of real-time PCR. The pilot scale validation of RT-PCR DNAzyme-based sensor has shown ~100% sensitivity and specificity in clinical specimens (nasopharyngeal swab, n = 34), with a good correlation (Spearman r = 0.799) with the Ct-value of fluorescence probe-based real-time PCR. These findings clearly indicate the potential of this inexpensive, sensitive, and specific molecular diagnostic test to extend our testing capabilities for the detection of SARS-CoV-2 to curtail COVID-19 transmission. |
| Databáze: | OpenAIRE |
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