Modeling Hypoxic Stress In Vitro Using Human Embryonic Stem Cells Derived Cardiomyocytes Matured by FGF4 and Ascorbic Acid Treatment
| Autor: | Ji-Na Park, Seung-Cheol Choi, Yongdoo Park, Ji-Hyun Choi, Soon Jun Hong, Jong-Hoon Kim, Hyojin Kim, Chi-Yeon Park, Dong-Hyung Kim, Long-Hui Cui, Jong Il Choi, Yongjun Jang, Tae Hee Ko, Kyung-Seob Kim, Myeong-Hwa Song, Ha-Rim Seo, Hyung Joon Joo, Ji-Min Noh, Jong-Ho Kim, Do Sun Lim, Se-Hwan Paek |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mesoderm
cardiac QH301-705.5 Heart Ventricles Human Embryonic Stem Cells Fibroblast Growth Factor 4 Myocardial Infarction Ascorbic Acid Biology Models Biological Article Cell Line Transcriptome Stress Physiological health services administration FGF4 medicine Humans Myocytes Cardiac Biology (General) Induced pluripotent stem cell health care economics and organizations hypoxia Cell Differentiation General Medicine differentiation Hypoxia (medical) Ascorbic acid Embryonic stem cell In vitro Cell Hypoxia cytokines Cell biology Culture Media medicine.anatomical_structure Gene Expression Regulation embryonic structures medicine.symptom pluripotent stem cells Biomarkers |
| Zdroj: | Cells, Vol 10, Iss 2741, p 2741 (2021) Cells Volume 10 Issue 10 |
| ISSN: | 2073-4409 |
| Popis: | Mature cardiomyocytes (CMs) obtained from human pluripotent stem cells (hPSCs) have been required for more accurate in vitro modeling of adult-onset cardiac disease and drug discovery. Here, we found that FGF4 and ascorbic acid (AA) induce differentiation of BG01 human embryonic stem cell–cardiogenic mesoderm cells (hESC-CMCs) into mature and ventricular CMs. Co-treatment of BG01 hESC-CMCs with FGF4+AA synergistically induced differentiation into mature and ventricular CMs. FGF4+AA-treated BG01 hESC-CMs robustly released acute myocardial infarction (AMI) biomarkers (cTnI, CK-MB, and myoglobin) into culture medium in response to hypoxic injury. Hypoxia-responsive genes and potential cardiac biomarkers proved in the diagnosis and prognosis of coronary artery diseases were induced in FGF4+AA-treated BG01 hESC-CMs in response to hypoxia based on transcriptome analyses. This study demonstrates that it is feasible to model hypoxic stress in vitro using hESC-CMs matured by soluble factors. |
| Databáze: | OpenAIRE |
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