A Comparison of the Anti-Tumor Effects of a Chimeric versus Murine Anti-CD19 Immunotoxins on Human B Cell Lymphoma and Pre-B Acute Lymphoblastic Leukemia Cell Lines
| Autor: | Ellen S. Vitetta, Lydia K. Tsai, Xiaoyun Liu, Laurentiu M. Pop |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Health
Toxicology and Mutagenesis medicine.medical_treatment Antigens CD19 lcsh:Medicine Antineoplastic Agents Mice SCID Ricin Pre-B Acute Lymphoblastic Leukemia ricin A chain Toxicology Article CD19 Mice Immunotoxin Cell Line Tumor Precursor B-Cell Lymphoblastic Leukemia-Lymphoma anti-CD19 medicine Animals Humans Cytotoxic T cell chimerization B cell Anti-CD19 Immunotoxins B-Lymphocytes biology Immunotoxins lcsh:R Antibodies Monoclonal U937 Cells Immunotherapy medicine.disease Burkitt Lymphoma Lymphoma medicine.anatomical_structure Immunology biology.protein Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | Toxins Volume 3 Issue 4 Pages 409-419 Toxins, Vol 3, Iss 4, Pp 409-419 (2011) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins3040409 |
| Popis: | Precursor B cell acute lymphoblastic leukemia (pre-B ALL) affects five to six thousand adults and almost three thousand children every year. Approximately 25% of the children and 60% of the adults die from their disease, highlighting the need for new therapies that complement rather than overlap chemotherapy and bone marrow transplantation. Immunotherapy is a class of therapies where toxicities and mechanisms of action do not overlap with those of chemotherapy. Because CD19 is a B cell- restricted membrane antigen that is expressed on the majority of pre-B tumor cells, a CD19-based immunotherapy is being developed for ALL. In this study, the anti-tumor activities of immunotoxins (ITs) constructed by conjugating a murine monoclonal antibody (MAb), HD37, or its chimeric (c) construct to recombinant ricin toxin A chain (rRTA) were compared both in vitro using human pre-B ALL and Burkitt’s lymphoma cell lines and in vivo using a disseminated human pre-B ALL tumor cell xenograft model. The murine and chimeric HD37 IT constructs were equally cytotoxic to pre-B ALL and Burkitt’s lymphoma cells in vitro and their use in vivo resulted in equivalent increases in survival of SCID mice with human pre-B ALL tumors when compared with control mice. |
| Databáze: | OpenAIRE |
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